Fatty Acid Synthase Recombinant Rabbit Monoclonal Antibody [JJ0939] (ET1701-91)
Catalog# ET1701-91
Rabbit Monoclonal to Fatty Acid Synthase
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
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Mouse
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Rat
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Fatty Acid Synthase antibody (ET1701-91) at 1/200 dilution and competitor's antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Fatty Acid Synthase Recombinant Rabbit Monoclonal Antibody [JJ0939] (ET1701-91)
Immunogen
Synthetic peptide within c-terminal human fatty acid synthase.
Host
Rabbit
Positive Control
HeLa, HeLa cell lysate, HEK-293 cell lysate, A549 cell lysate, C2C12 cell lysate, L-929 cell lysate, SK-Br-3, A549, RH-35, MCF-7, SW480, human breast carcinoma tissue, mouse colon tissue, human liver tissue, HeLa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ0939
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:100-1:500
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IF-Tissue
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1:100-1:500
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IHC-P
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1:50-1:200
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 273 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Fatty Acid Synthase
SYNONYMS
[Acyl-carrier-protein] S acetyltransferase antibody;[Acyl-carrier-protein] S malonyltransferase antibody;3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody;3-oxoacyl-[acyl-carrier-protein] reductase antibody;3-oxoacyl-[acyl-carrier-protein] synthase antibody;Enoyl-[acyl-carrier-protein] reductase antibody;FAS antibody;FAS_HUMAN antibody;FASN antibody;Fatty acid synthase antibody;MGC14367 antibody;MGC15706 antibody;OA 519 antibody;Oleoyl-[acyl-carrier-protein] hydrolase antibody;SDR27X1 antibody;Short chain dehydrogenase/reductase family 27X member 1 antibody
TISSUE SPECIFICITY
Ubiquitous. Prominent expression in brain, lung, and liver.
SUBCELLULAR LOCATION
Cytoplasm, Melanosome.
FUNCTION
Fatty acid biosynthesis is mediated by seven catalytic enzymes and an acyl carrier protein (ACP), to which various acyl intermediates are covalently attached. Fatty Acid Synthase (FAS) is the anabolic enzyme that contains the seven unique catalytic sites and mediates the conversion of acetyl-CoA and malonyl-CoA, in the presence of the cofactor NADPH, into long-chain saturated fatty acids, such as palmitate. Human Fatty Acid Synthase cDNA encodes a 2,504 amino acid protein. Catalytically active Fatty Acid Synthase is a homodimer. Human Fatty Acid Synthase mRNA is variably expressed with abundant levels present in brain, lung and liver. Fatty acid synthetic metabolism is abnormally elevated in tumor cells and may support cell growth or survival of malignant cancers.
CITATIONS
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Sun Mingjun,et al
Synergism of Fusobacterium periodonticum and N-nitrosamines promote the formation of EMT subtypes in ESCC by modulating Wnt3a palmitoylation
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IHC
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Mouse
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Citation
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Chen, P., Li, S., Zhou, Z., Wang, X., Shi, D., Li, Z., Li, X., & Xiao, Y.
Liver fat metabolism of broilers regulated by Bacillus amyloliquefaciens TL via stimulating IGF-1 secretion and regulating the IGF signaling pathway
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WB
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Broiler
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Citation
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Wu Peiyao,et al
Periodontal pathogen Fusobacterium nucleatum infection accelerates hepatic steatosis in high‐fat diet‐fed ApoE knockout mice by inhibiting Nrf2/Keap1 signaling
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WB
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IHC
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Mouse
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Citation
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Brexpiprazole suppresses cell proliferation and de novo lipogenesis through AMPK/SREBP1 pathway in colorectal cancer
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WB
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Human
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Citation
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