GAPDH Mouse Monoclonal Antibody [5-E10] (EM1101)
Catalog# EM1101
Mouse Monoclonal to GAPDH
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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Zebrafish
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Rabbit
Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: SK-Br-3 cell lysate (20 µg/Lane)
Lane 5: JAR cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Lane 7: C2C12 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: Mouse kidney tissue lysate (40 µg/Lane)
Lane 10: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1101) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
GAPDH Mouse Monoclonal Antibody [5-E10] (EM1101)
Immunogen
Synthetic peptide within human gapdh aa 10-50.
Host
Mouse
Positive Control
MDA-MB-231 cell lysate, HeLa cell lysate, MCF7 cell lysate, SK-Br-3 cell lysate, JAR cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, Rat liver tissue lysate, HeLa, F9, zebrafish cell/tissue lysates, human liver tissue, mouse liver tissue, human spleen tissue, rat liver tissue, rat spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
5-E10
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:10,000
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IF-Cell
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1:200-1:500
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IHC-P:1:200-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein L affinity purified.
Molecular Weight
Predicted band size: 36 kDa
Isotype
IgM
TARGET
UNIPROT #
PROTEIN NAME
GAPDH
SYNONYMS
38 kDa BFA-dependent ADP-ribosylation substrate antibody;aging associated gene 9 protein antibody;Aging-associated gene 9 protein antibody;BARS-38 antibody;cb609 antibody;EC 1.2.1.12 antibody;Epididymis secretory sperm binding protein Li 162eP antibody;G3P_HUMAN antibody;G3PD antibody;G3PDH antibody;GAPD antibody;GAPDH antibody;Glyceraldehyde 3 phosphate dehydrogenase antibody;Glyceraldehyde-3-phosphate dehydrogenase antibody;HEL-S-162eP antibody;KNC-NDS6 antibody;MGC102544 antibody;MGC102546 antibody;MGC103190 antibody;MGC103191 antibody;MGC105239 antibody;MGC127711 antibody;MGC88685 antibody;OCAS, p38 component antibody;OCT1 coactivator in S phase, 38-KD component antibody;peptidyl cysteine S nitrosylase GAPDH antibody;Peptidyl-cysteine S-nitrosylase GAPDH antibody;wu:fb33a10 antibody
SEQUENCE SIMILARITIES
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
POST-TRANSLATIONAL MODIFICATION
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity.; ISGylated.; Sulfhydration at Cys-152 increases catalytic activity.; Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation.; Succination of Cys-152 and Cys-247 by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits glyceraldehyde-3-phosphate dehydrogenase activity. Fumarate concentration as well as succination of cysteine residues in GAPDH is significantly increased in muscle of diabetic mammals. It was proposed that the S-(2-succinyl)cysteine chemical modification may be a useful biomarker of mitochondrial and oxidative stress in diabetes and that succination of GAPDH and other thiol proteins by fumarate may contribute to the metabolic changes underlying the development of diabetes complications.
SUBCELLULAR LOCATION
Cytoplasm
FUNCTION
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
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Wang, H., Li, T., Wang, X., Guan, Y., Jiang, Y., Chen, S., Zou, S., & Duan, P.
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WB
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Protective effect of cultured bear bile powder against dimethylnitrosamine-induced hepatic fibrosis in rats.
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WB
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rat
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Liu Xinghui,et al
Exercise following joint distraction inhibits muscle wasting and delays the progression of post-traumatic osteoarthritis in rabbits by activating PGC-1α in skeletal …
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WB
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Human
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Xuewen Gao
FvSet2 regulates fungal growth,pathogenicity,and secondary metabolism in Fusarium verticillioides
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WB
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Fusarium verticillioides
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Gemfibrozil improves lipid metabolism in Nile tilapia Oreochromis niloticus fed a high-carbohydrate diet through peroxisome proliferator activated receptor-α activation
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WB
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Nile tilapia
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Qingfeng Tang
Characterization of the Expression and Functions of Two Odorant-Binding Proteins of Sitophilus zeamais Motschulsky (Coleoptera: Curculionoidea)
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WB
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Sitophilus zeamais Motschulsky
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Gu, Q., Wang, Y., Zhao, X., Yuan, B., Zhang, M., Tan, Z., Zhang, X., Chen, Y., Wu, H., Luo, Y., Keller, N. P., Gao, X., & Ma, Z.
Inhibition of histone acetyltransferase GCN5 by a transcription factor FgPacC controls fungal adaption to host-derived iron stress
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WB
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