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iFluor™ 594 Conjugated Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05]

Usd: 429

Specification

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Catalog# HA720120F

iFluor™ 594 Conjugated Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05]

Application

  • IF-Cell

  • IF-Tissue

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • iFluor™ 594

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

iFluor™ 594 Conjugated Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Cytokeratin 7 aa 18-67.

Species Reactivity

Human, Mouse

Validated Applications

IF-Cell, IF-Tissue, FC

Molecular Weight

51 kDa

Positive Control

Human breast tissue, human liver tissue, SK-Br-3.

Conjugation

iFluor™ 594

Clone Number

ST50-05

RRID

AB_3072059

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Cell

  • 1:100

  • IF-Tissue

  • 1:200-1:500

  • FC

  • 1ug/mL

Target

Function

Keratin, type II cytoskeletal 7 also known as cytokeratin-7 (CK-7) or keratin-7 (K7) or sarcolectin (SCL) is a protein that in humans is encoded by the KRT7 gene.Keratin 7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. Keratin-7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.

Background References

1. Hrudka J. et al. Cytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma. Sci Rep. 2021 Sep

2. Statz E. et al. Cytokeratin 7, GATA3, and SOX-10 is a Comprehensive Panel in Diagnosing Triple Negative Breast Cancer Brain Metastases. Int J Surg Pathol. 2021 Aug

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P08729 Human

SwissProt: Q9DCV7 Mouse

Synonyms

CK 7 antibody

CK-7 antibody

CK7 antibody

Cytokeratin 7 antibody

Cytokeratin-7 antibody

D15Wsu77e antibody

K2C7 antibody

K2C7_HUMAN antibody

K7 antibody

Keratin 7 antibody

Expand

Images

  • Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (<a href="/products/HA720120F" style="font-weight: bold;text-decoration: underline;">HA720120F</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 7 (<a href="/products/HA720120F" style="font-weight: bold;text-decoration: underline;">HA720120F</a>, iFluor&trade; 594) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (HA720120F).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 7 (HA720120F, iFluor™ 594) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

  • Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 7 (<a href="/products/HA720120F" style="font-weight: bold;text-decoration: underline;">HA720120F</a>) and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (<a href="/products/HA720120F" style="font-weight: bold;text-decoration: underline;">HA720120F</a>, red) at 1/200 dilution and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>, green) at 1/500 dilution at +4℃ overnight, washed with PBS.<br /><br />Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 7 (HA720120F) and Vimentin (EM0401).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (HA720120F, red) at 1/200 dilution and Vimentin (EM0401, green) at 1/500 dilution at +4℃ overnight, washed with PBS.

    Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 7 (<a href="/products/HA720120F" style="font-weight: bold;text-decoration: underline;">HA720120F</a>).<br /><br />Cells were fixed in methanol and then blocked with 2% negative goat serum for 15 minutes at room temperature. The cells were then incubated overnight at +4℃ with Cytokeratin 7 (<a href="/products/HA720120F" style="font-weight: bold;text-decoration: underline;">HA720120F</a>, red) at 1/100 dilution. Nuclei were counterstained with DAPI (blue).

    Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 7 (HA720120F).

    Cells were fixed in methanol and then blocked with 2% negative goat serum for 15 minutes at room temperature. The cells were then incubated overnight at +4℃ with Cytokeratin 7 (HA720120F, red) at 1/100 dilution. Nuclei were counterstained with DAPI (blue).

  • Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 7.<br /><br />Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 7 (<a href="/products/HA720120F" style="font-weight: bold;text-decoration: underline;">HA720120F</a>, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 7.

    Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 7 (HA720120F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation