Histone H1.2 Recombinant Rabbit Monoclonal Antibody [JU43-48] (ET1706-26)
Catalog# ET1706-26
Rabbit Monoclonal to Histone H1.2
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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ChIP
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Human
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Mouse
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Rat
Lane 1: Hela cell lysate
Lane 2: 293 cell lysate
Lane 3: MCF-7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 25 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-26) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 5,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Histone H1.2 Recombinant Rabbit Monoclonal Antibody [JU43-48] (ET1706-26)
Immunogen
Synthetic peptide within human histone h12 aa 1-50 / 213.
Host
Rabbit
Positive Control
Hela cell lysate, 293 cell lysate, MCF-7 cell lysate, rat liver tissue lysate, mouse lung tissue lysate, Hela, PC-3M, SK-Br-3, mouse colon tissue, human kidney tissue, rat brain tissue, human lung carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU43-48
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:500
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IF-Tissue
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1:50-1:100
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IHC-P
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1:50-1:200
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ChIP
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Use 0.5~2 μg for 25 μg of chromatin.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
21 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Histone H1.2
SYNONYMS
H1 histone family member 2 antibody;H1.a antibody;H12_HUMAN antibody;H1F2 antibody;H1s-1 antibody;HIST1H1C antibody;Histone 1 H1c antibody;Histone cluster 1 H1c antibody;Histone H1.2 antibody;Histone H1c antibody;Histone H1d antibody;Histone H1s-1 antibody;MGC3992 antibody
SEQUENCE SIMILARITIES
Belongs to the histone H1/H5 family.
POST-TRANSLATIONAL MODIFICATION
H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter.; Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.; Citrullination at Arg-54 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.; ADP-ribosylated on Ser-188 in response to DNA damage.
SUBCELLULAR LOCATION
Nucleus. Chromosome.
FUNCTION
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
CITATIONS
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Song Yangran,et al
Histone H1. 2 Inhibited EMCV Replication through Enhancing MDA5-Mediated IFN-β Signaling Pathway
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WB
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Human
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Citation
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