HNF-4-alpha Recombinant Rabbit Monoclonal Antibody [SN72-03] (ET1611-43)
Catalog# ET1611-43
Rabbit Monoclonal to HNF-4-alpha
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
HNF-4-alpha Recombinant Rabbit Monoclonal Antibody [SN72-03] (ET1611-43)
Immunogen
Synthetic peptide within human hnf-4-alpha aa 21-70 / 474.
Host
Rabbit
Positive Control
A549 cell lysates, LOVO cell lysates, HepG2, SW480, human colon cancer tissue, human small intestine tissue, mouse colon tissue, rat colon tissue, Mouse liver tissue lysate, Mouse kidney tissue lysate, Mouse brain tissue lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN72-03
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:100
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IHC-P
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1:200-1:3,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 53 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
HNF-4-alpha
SYNONYMS
FLJ39654 antibody;FRTS4 antibody;Hepatic nuclear factor 4 alpha antibody;Hepatocyte nuclear factor 4 alpha antibody;Hepatocyte nuclear factor 4 antibody;Hepatocyte nuclear factor 4-alpha antibody;HNF 4 alpha antibody;HNF 4 antibody;HNF-4-alpha antibody;HNF4 antibody;HNF4A antibody;HNF4A_HUMAN antibody;HNF4a7 antibody;HNF4a8 antibody;HNF4a9 antibody;Hnf4alpha antibody;HNF4alpha10/11/12 antibody;MODY 1 antibody;MODY antibody;MODY1 antibody;NR2A1 antibody;NR2A21 antibody;Nuclear receptor subfamily 2 group A member 1 antibody;OTTHUMP00000031060 antibody;OTTHUMP00000031062 antibody;TCF 14 antibody;TCF antibody;TCF-14 antibody;TCF14 antibody;Tcf4 antibody;Transcription factor 14, hepatic nuclear factor antibody;Transcription factor 14 antibody;Transcription factor HNF 4 antibody;Transcription factor HNF-4 antibody;Transcription factor HNF4 antibody
SEQUENCE SIMILARITIES
Belongs to the nuclear hormone receptor family. NR2 subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.; Acetylation at Lys-458 lowers transcriptional activation by about two-fold.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
HNF-4 alpha is a transcription factor that binds DNA as a homodimer. HNF-4 alpha is important in liver, kidney, and intestinal development. It has also been intensely studied as one of a variety of genes responsible for diabetes mellitus. HNF-4 alpha has been shown in knock out mice to be essential for the morphogenic and functional differentiation of hepatocytes. HNF-4 alpha is a dominant regulator of epithelial phenotypes able to drive the mesenchymal-to-epithelial transition when expressed in fibroblasts.
CITATIONS
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Shu, Y., Xu, Q., Xu, Y., Tao, Q., Shao, M., Cao, X., Chen, Y., Wu, Z., Chen, M., Zhou, Y., Zhou, P., Shi, Y., & Bu, H.
Loss of Numb promotes hepatic progenitor expansion and intrahepatic cholangiocarcinoma by enhancing Notch signaling
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IHC
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Mouse
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Citation
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