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Human IgG heavy chain Mouse Monoclonal Antibody [54-76]

Usd: 350

Specification

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Catalog# M0806-1

Human IgG heavy chain Mouse Monoclonal Antibody [54-76]

Application

  • WB

  • ELISA

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Human IgG heavy chain Mouse Monoclonal Antibody [54-76]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Native protein.

Species Reactivity

Human

Validated Applications

WB, ELISA

Molecular Weight

50 kDa

Positive Control

Human IgG protein lysates.

Conjugation

unconjugated

Clone Number

54-76

RRID

AB_3072987

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • ELISA

  • 1:5,000

Target

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens . The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen .

Background References

1. Teng G.et.al.Immunoglobulin somatic hypermutation.Annu. Rev. Genet. 41:107-120(2007).

Subcellular Location

Cell membrane, Immunoglobulin, Membrane, Secreted.

UNIPROT

SwissProt: P01857 Human

SwissProt: P01859 Human

SwissProt: P01860 Human

SwissProt: P01861 Human

Synonyms

FLJ39988 antibody

FLJ40036 antibody

FLJ40253 antibody

FLJ40587 antibody

FLJ40789 antibody

FLJ40834 antibody

G1m marker antibody

G2m marker antibody

G3m marker antibody

G4m marker antibody

Expand

Images

  • Western blot analysis of Human IgG heavy chain on human IgG protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/M0806-1" style="font-weight: bold;text-decoration: underline;">M0806-1</a>, 1/5000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Human IgG heavy chain on human IgG protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0806-1, 1/5000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation