Ikaros Recombinant Rabbit Monoclonal Antibody [JB50-38] (ET7107-25)

UNIPROT #

Q13422

PROTEIN NAME

Ikaros

SYNONYMS

CLL associated antigen KW 6 antibody;DNA-binding protein Ikaros antibody;hIk 1 antibody;Hs.54452 antibody;IK1 antibody;Ikaros (zinc finger protein) antibody;IKAROS antibody;IKAROS family zinc finger 1 (Ikaros) antibody;Ikaros family zinc finger protein 1 antibody;Ikzf1 antibody;IKZF1_HUMAN antibody;LYF1 antibody;Lymphoid transcription factor LyF-1 antibody;PRO0758 antibody;Zinc finger protein subfamily 1A 1 (Ikaros) antibody;Zinc finger protein subfamily 1A 1 antibody;Zinc finger protein, subfamily 1A, member 1 antibody;ZNFN1A1 antibody

SEQUENCE SIMILARITIES

Belongs to the Ikaros C2H2-type zinc-finger protein family.

TISSUE SPECIFICITY

Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation controls cell-cycle progression from late G(1) stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G(1) phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues (By similarity). Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation.; Sumoylated. Simulataneous sumoylation on the 2 sites results in a loss of both HDAC-dependent and HDAC-independent repression. Has no effect on pericentromeric heterochromatin location. Desumoylated by SENP1 (By similarity).; Polyubiquitinated.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Ikaros is a transcription factor that is encoded by the IKZF genes of the Ikaros family zinc finger group. Zinc finger is a small structural motif of protein that allows protein binding to DNA or RNA molecule that is characterized by the coordination of one or more zinc ions (Zn2+) in order to stabilize the fold. Ikaros displays crucial functions in the hematopoietic system and is a known regulator of immune cells development, mainly in early B cells, CD4+ T cells. Its dysfunction has been linked to the development of chronic lymphocytic leukemia. In particular, Ikaros has been found in recent years to be a major tumor suppressor involved in human B-cell acute lymphoblastic leukemia and that it also has a part in the differentiation and function of individual T helper cells. Ikaros also has a role during the later stages of B cell development during VDJ recombination in switch class of the antibody isotypes and expression of the B cell receptor. SNPs located near the 3' region of IKZF1 in humans have been linked to susceptibility to childhood acute lymphoblastic leukemia (ALL) as well as type 1 diabetes. The two effects appear to be in opposite directions, with the allele marking susceptibility to ALL protecting from T1D and vice versa.