Integrin alpha 2 Recombinant Rabbit Monoclonal Antibody [SN0752] (ET1611-57)
Catalog# ET1611-57
Rabbit Monoclonal to Integrin alpha 2
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WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1/2: Wild-type Hela whole cell lysate (20 µg).
Lane 3/4: Integrin alpha 2 fragment 1 knockdown Hela whole cell lysate (20 µg).
Lane 5/6: Integrin alpha 2 fragment 2 knockdown Hela whole cell lysate (20 µg).
ET1611-57 was shown to specifically react with Integrin alpha 2 in wild-type Hela cells. Weakened bands were observed when Integrin alpha 2 knockdown samples were tested. Wild-type and Integrin alpha 2 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1611-57, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Integrin alpha 2 Recombinant Rabbit Monoclonal Antibody [SN0752] (ET1611-57)
Immunogen
Synthetic peptide within human integrin alpha 2 aa 1,132-1,181 / 1,181.
Host
Rabbit
Positive Control
MCF7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, human colon carcinoma tissue, human breast carcinoma tissue, human kidney tissue, mouse colon tissue, mouse stomach tissue, A431.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN0752
RRID
APPLICATION DILUTION
-
WB
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1:5,000
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IF-Cell
-
1:100-1:500
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IF-Tissue
-
1:100-1:500
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IHC-P
-
1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 129 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Integrin alpha 2
SYNONYMS
BR antibody;CD 49b antibody;CD49 antigen like family member B antibody;CD49 antigen-like family member B antibody;CD49b antibody;CD49b antigen antibody;Collagen receptor antibody;DX5 antibody;Glycoprotein Ia deficiency included antibody;GP Ia antibody;GP Ia deficiency, included antibody;GPIa antibody;HPA 5 included antibody;HPA5 included antibody;Human platelet alloantigen system 5 antibody;Integrin alpha 2 antibody;Integrin alpha-2 antibody;Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody;ITA2_HUMAN antibody;ITGA2 antibody;Platelet alloantigen Br(a), included antibody;Platelet antigen Br antibody;Platelet glycoprotein GPIa antibody;Platelet glycoprotein Ia antibody;Platelet glycoprotein Ia/IIa antibody;Platelet membrane glycoprotein Ia antibody;Platelet receptor for collagen, deficiency of, included antibody;Very late activation protein 2 receptor alpha 2 subunit antibody;VLA 2 alpha chain antibody;VLA 2 antibody;VLA 2 subunit alpha antibody;VLA-2 subunit alpha antibody;VLA2 antibody;VLA2 receptor alpha 2 subunit antibody;VLAA2 antibody
SEQUENCE SIMILARITIES
Belongs to the integrin alpha chain family.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. The sixteen α and eight β subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as fibrinogen, or to counter-receptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. Integrin α2 is responsible for adhesion of platelets and other cells to collagens. Modulation of collagen and collagenase gene expression force generation and organization of newly synthesized extracellular matrix.
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