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KCNJ13/Kir7.1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500215

KCNJ13/Kir7.1 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

KCNJ13/Kir7.1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within C-terminal human KCNJ13/Kir7.1 from aa 200-aa360.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

41 kDa

Positive Control

Rat brain tissue lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, human small intestine tissue.

Conjugation

unconjugated

RRID

AB_3071312

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

Target

Function

This gene encodes a member of the inwardly rectifying potassium channel family of proteins. Members of this family form ion channel pores that allow potassium ions to pass into a cell. The encoded protein belongs to a subfamily of low signal channel conductance proteins that have a low dependence on potassium concentration. Mutations in this gene are associated with snowflake vitreoretinal degeneration. Alternate splicing results in multiple transcript variants.

Background References

1. Kanzaki Y. et. al. KCNJ13 Gene Deletion Impairs Cell Alignment and Phagocytosis in Retinal Pigment Epithelium Derived from Human-Induced Pluripotent Stem Cells. Invest Ophthalmol Vis Sci. 2020 May

2. Roman D. et. al. Conditional loss of Kcnj13 in the retinal pigment epithelium causes photoreceptor degeneration. Exp Eye Res. 2018 Nov

Subcellular Location

Membrane.

UNIPROT

SwissProt: O60928 Human

SwissProt: P86046 Mouse

SwissProt: O70617 Rat

Synonyms

Inward rectifier potassium channel 13

Inward rectifier K(+) channel Kir7.1

Potassium channel, inwardly rectifying subfamily J member 13

KCNJ13

Kir7.1

Images

  • Western blot analysis of KCNJ13/Kir7.1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA500215" style="font-weight: bold;text-decoration: underline;">HA500215</a>, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: Rat brain tissue lysate<br />Lane 2: Mouse brain tissue lysate<br />Lane 3: Mouse cerebellum tissue lysate

    Western blot analysis of KCNJ13/Kir7.1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500215, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Rat brain tissue lysate
    Lane 2: Mouse brain tissue lysate
    Lane 3: Mouse cerebellum tissue lysate

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-KCNJ13/Kir7.1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500215" style="font-weight: bold;text-decoration: underline;">HA500215</a>, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-KCNJ13/Kir7.1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500215, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"