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Claudin 7 Mouse Monoclonal Antibody [6-G3]

Usd: 350

Specification

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Catalog# M1412-2

Claudin 7 Mouse Monoclonal Antibody [6-G3]

Application

  • WB

  • IF-Cell

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Claudin 7 Mouse Monoclonal Antibody [6-G3]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Human Claudin 7 aa 162-211 / 211.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 22 kDa

Positive Control

SW480 cell lysate, MCF7 cell lysate, LNCaP cell lysate, Caco-2 cell lysate, A431 cell lysate, mouse colon tissue lysate, rat colon tissue lysate, PC-12, human kidney tissue, mouse colon tissue, rat colon tissue.

Conjugation

unconjugated

Clone Number

6-G3

RRID

AB_3073091

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:5,000

  • IF-Tissue

  • 1:200-1:1,000

Target

Function

Claudin-7 is a protein that in humans is encoded by the CLDN7 gene. It belongs to the group of claudins.Claudins, such as CLDN7, are involved in the formation of tight junctions between epithelial cells. Tight junctions restrict lateral diffusion of lipids and membrane proteins, and thereby physically define the border between the apical and basolateral compartments of epithelial cells. It is expressed in kidney, lung and prostate. Claudin-7 is down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.

Background References

1. "Regulation of the expression of the prostate-specific antigen by claudin-7." Zheng J.-Y., Yu D., Foroohar M., Ko E., Chan J., Kim N., Chiu R., Pang S. J. Membr. Biol. 194:187-197(2003)

2. "Loss of the tight junction protein claudin-7 correlates with histological grade in both ductal carcinoma in situ and invasive ductal carcinoma of the breast." Kominsky S.L., Argani P., Korz D., Evron E., Raman V., Garrett E., Rein A., Sauter G., Kallioniemi O.-P., Sukumar S.Oncogene 22:2021-2033(2003)

3. "The cell-cell adhesion molecule EpCAM interacts directly with the tight junction protein claudin-7." Ladwein M., Pape U.F., Schmidt D.S., Schnoelzer M., Fiedler S., Langbein L., Franke W.W., Moldenhauer G., Zoeller M. Exp. Cell Res. 309:345-357(2005)

Sequence Similarity

Belongs to the claudin family.

Tissue Specificity

Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.

Post-translational Modification

Phosphorylated.

Subcellular Location

Cell membrane, Basolateral cell membrane, Cell junction, tight junction.

UNIPROT

SwissProt: O95471 Human

SwissProt: Q9Z261 Mouse

SwissProt: Q9Z1L1 Rat

Synonyms

CEPTR L2 antibody

CEPTRL 2 antibody

CEPTRL2 antibody

Claudin 1 antibody

Claudin 9 antibody

Claudin-7 antibody

Claudin1 antibody

Claudin7 antibody

Claudin9 antibody

CLD7_HUMAN antibody

Expand

Images

  • Western blot analysis of Claudin 7 on different lysates with Mouse anti-Claudin 7 antibody (<a href="/products/M1412-2" style="font-weight: bold;text-decoration: underline;">M1412-2</a>) at 1/1,000 dilution.<br /><br />Lane 1: SW480 cell lysate (20 µg/Lane)<br />Lane 2: MCF7 cell lysate (20 µg/Lane)<br />Lane 3: LNCaP cell lysate (20 µg/Lane)<br />Lane 4: Caco-2 cell lysate (20 µg/Lane)<br />Lane 5: A431 cell lysate (20 µg/Lane)<br />Lane 6: Mouse colon tissue lysate (40 µg/Lane)<br />Lane 7: Rat colon tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 22 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1412-2" style="font-weight: bold;text-decoration: underline;">M1412-2</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Claudin 7 on different lysates with Mouse anti-Claudin 7 antibody (M1412-2) at 1/1,000 dilution.

    Lane 1: SW480 cell lysate (20 µg/Lane)
    Lane 2: MCF7 cell lysate (20 µg/Lane)
    Lane 3: LNCaP cell lysate (20 µg/Lane)
    Lane 4: Caco-2 cell lysate (20 µg/Lane)
    Lane 5: A431 cell lysate (20 µg/Lane)
    Lane 6: Mouse colon tissue lysate (40 µg/Lane)
    Lane 7: Rat colon tissue lysate (40 µg/Lane)

    Predicted band size: 22 kDa
    Observed band size: 20 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1412-2) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Claudin 7 antibody (<a href="/products/M1412-2" style="font-weight: bold;text-decoration: underline;">M1412-2</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1412-2" style="font-weight: bold;text-decoration: underline;">M1412-2</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Claudin 7 antibody (M1412-2) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1412-2) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-Claudin 7 antibody (<a href="/products/M1412-2" style="font-weight: bold;text-decoration: underline;">M1412-2</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1412-2" style="font-weight: bold;text-decoration: underline;">M1412-2</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-Claudin 7 antibody (M1412-2) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1412-2) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-Claudin 7 antibody (<a href="/products/M1412-2" style="font-weight: bold;text-decoration: underline;">M1412-2</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1412-2" style="font-weight: bold;text-decoration: underline;">M1412-2</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-Claudin 7 antibody (M1412-2) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1412-2) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Claudin 7 with Mouse anti-Claudin 7 antibody (<a href="/products/M1412-2" style="font-weight: bold;text-decoration: underline;">M1412-2</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/M1412-2" style="font-weight: bold;text-decoration: underline;">M1412-2</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Claudin 7 with Mouse anti-Claudin 7 antibody (M1412-2) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (M1412-2, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunocytochemistry analysis of PC-12 cells labeling Claudin 7 with Mouse anti-Claudin 7 antibody (<a href="/products/M1412-2" style="font-weight: bold;text-decoration: underline;">M1412-2</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Claudin 7 antibody (<a href="/products/M1412-2" style="font-weight: bold;text-decoration: underline;">M1412-2</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of PC-12 cells labeling Claudin 7 with Mouse anti-Claudin 7 antibody (M1412-2) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Claudin 7 antibody (M1412-2) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

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Claudin 7 Recombinant Mouse Monoclonal Antibody [6-G3-R]

Application: WB,IF-Cell,IHC-P,IF-Tissue,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated