Mammaglobin A Recombinant Rabbit Monoclonal Antibody [PD00-74]
Catalog# HA721202
Mammaglobin A Recombinant Rabbit Monoclonal Antibody [PD00-74]
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
Mammaglobin A Recombinant Rabbit Monoclonal Antibody [PD00-74]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within C terminal Human Mammaglobin A.
Species Reactivity
Human
Validated Applications
IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 10 kDa
Positive Control
Human skin tissue, human breast tissue.
Conjugation
unconjugated
Clone Number
PD00-74
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:2,000
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IF-Tissue
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1:1,000
Target
Function
Mammaglobin-A also known as secretoglobin family 2A member 2 is a protein that in humans is encoded by the SCGB2A2 gene. SCGB2A2 is a member of the superfamily of secretoglobins, a group of small dimeric secreted and sometimes glycosylated proteins. Expressed mainly in mucosa, secretoglobins seem to be involved in cell signalling, immune response, and chemotaxis, and may also serve as transporters for steroid hormones in humans. SCGB2A2 expression is highly specific of mammary tissue, and is increasingly used for identification and detection of disseminated breast cancer cells. Mammaglobin is breast-associated glycoprotein distantly related to secretoglobin family that includes human uteroglobin and lipophilin. Anti-mammaglobin labels cytoplasm of normal breast epithelial cells as well as primary and metastatic breast carcinomas. Absence of mammaglobin expression is typically seen in prostate, kidney, colon, rectum, small intestine, stomach, pancreas, lung and thyroid tissue. Mammaglobin may be used as part of an immunohistochemical panel for determination of metastatic breast carcinoma and tumor of unknown primary origin.
Background References
1. Liu Z et al. Identification and characterization of mammaglobin-A epitope in heterogenous breast cancers for enhancing tumor-targeting therapy. Signal Transduct Target Ther. 2020 May
2. Talaat IM et al. Bone marrow mammaglobin-1 (SCGB2A2) immunohistochemistry expression as a breast cancer specific marker for early detection of bone marrow micrometastases. Sci Rep. 2020 Aug
Subcellular Location
Secreted.
UNIPROT
Synonyms
Mammaglobin 1 antibody
Mammaglobin-1 antibody
Mammaglobin-A antibody
MGB1 antibody
Scgb2a2 antibody
Secretoglobin family 2A member 2 antibody
SG2A2 antibody
SG2A2_HUMAN antibody
UGB2 antibody
Images
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Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Mammaglobin A antibody (HA721202) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721202) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Mammaglobin A antibody (HA721202) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721202) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: breast
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"