MyD88 Recombinant Rabbit Monoclonal Antibody [SC65-04] (ET1610-81)
Catalog# ET1610-81
Rabbit Monoclonal to MyD88
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
FC
-
Human
Lane 1: HepG2 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 35 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-81) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
MyD88 Recombinant Rabbit Monoclonal Antibody [SC65-04] (ET1610-81)
Immunogen
Synthetic peptide within human myd88 aa 1-49 / 491.
Host
Rabbit
Positive Control
HepG2 cell lysate, MCF7 cell lysate, HeLa cell lysate, HepG2, MCF7, human tonsil tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC65-04
RRID
APPLICATION DILUTION
-
WB
-
1:1,000
-
IF-Cell
-
1:100-1:250
-
IF-Tissue
-
1:100-1:500
-
IHC-P
-
1:50-1:200
-
FC
-
1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 33 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MyD88
SYNONYMS
Mutant myeloid differentiation primary response 88 antibody;MYD 88 antibody;Myd88 antibody;MYD88_HUMAN antibody;MYD88D antibody;Myeloid differentiation marker 88 antibody;Myeloid differentiation primary response 88 antibody;Myeloid differentiation primary response gene (88) antibody;Myeloid differentiation primary response gene 88 antibody;Myeloid differentiation primary response gene antibody;Myeloid differentiation primary response protein MyD88 antibody;OTTHUMP00000161718 antibody;OTTHUMP00000208595 antibody;OTTHUMP00000209058 antibody;OTTHUMP00000209059 antibody;OTTHUMP00000209060 antibody
TISSUE SPECIFICITY
Ubiquitous.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination. OTUD4 specifically hydrolyzes 'Lys-63'-linked polyubiquitinated MYD88.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Interleukin-1 (IL-1)-induced activation of the NFκB pathway is mediated through the IL-1 receptor and the subsequent phosphorylation of IL-1 receptor-associated kinase (IRAK). The myeloid differentiation protein MyD88 was originally characterized as a protein upregulated in myeloleukemic cells following IL-6-induced growth arrest and terminal differentiation. MyD88 is now known to function as an adaptor protein for the association of IRAK with the IL-1 receptor. MyD88 is functionally homologous to the adaptor protein tube in the Toll signaling pathway of Drosophilia, and both proteins are members of the Toll/IL-1R superfamily. MyD88 contains a characteristic N-terminal death domain that is essential for NFκB activation and an adjacent Toll/IL-1R homology domain (TIR domain). Collectively, these domains enable the protein-protein interactions of MyD88 with IRAK and the IL-1 receptor complex.
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