NDUFB8 Recombinant Rabbit Monoclonal Antibody [JG61-36] (ET7108-25)
Catalog# ET7108-25
Rabbit Monoclonal to NDUFB8
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WB
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IP
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: C6 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 19 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-25) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
NDUFB8 Recombinant Rabbit Monoclonal Antibody [JG61-36] (ET7108-25)
Immunogen
Recombinant protein within human ndufb8 aa 1-186 / 186.
Host
Rabbit
Positive Control
HeLa cell lysate, A549 cell lysate, RAW264.7 cell lysate, C6 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, HeLa, C6, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG61-36
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IP
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1-2μg/sample
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IHC-P
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1:200
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IF-Cell
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 22 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
NDUFB8
SYNONYMS
ASHI antibody;CI-ASHI antibody;Complex I ASHI subunit antibody;Complex I-ASHI antibody;mitochondrial antibody;NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody;NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody;NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody;NADH-ubiquinone oxidoreductase ASHI subunit antibody;NDUB8_HUMAN antibody;NDUFB8 antibody
SEQUENCE SIMILARITIES
Belongs to the complex I NDUFB8 subunit family.
SUBCELLULAR LOCATION
Mitochondrion.
FUNCTION
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
CITATIONS
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Wang Yi,et al
Nanoengineered mitochondria enable ocular mitochondrial disease therapy via the replacement of dysfunctional mitochondria
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WB
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Mouse
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Citation
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-
Jin, X., Li, L., Peng, Q., Gan, C., Gao, L., He, S., Tan, S., Pu, W., Liu, Y., Gong, Y., Yao, Y., Wang, G., Liu, X., Gong, M., Lei, P., Zhang, H., Qi, S., Xu, H., Hu, H., Dong, B., … Dai, L.
Glycyrrhetinic acid restricts mitochondrial energy metabolism by targeting SHMT2
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WB
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Human
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Citation
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-
Yi Wang,et al
Mitophagy-Enhanced Nanoparticle-Engineered Mitochondria Restore Homeostasis of Mitochondrial Pool for Alleviating Pulmonary Fibrosis
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WB
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Mouse
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Citation
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