NQO2 Recombinant Rabbit Monoclonal Antibody [SC06-68]
Catalog# ET1610-92
NQO2 Recombinant Rabbit Monoclonal Antibody [SC06-68]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
NQO2 Recombinant Rabbit Monoclonal Antibody [SC06-68]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human NQO2 aa 2-49 / 231.
Species Reactivity
Human (Predicted: Mouse, Rat)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 26 kDa
Positive Control
Hela cell lysates, human kidney tissue.
Conjugation
unconjugated
Clone Number
SC06-68
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IHC-P
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1:50-1:200
Target
Function
NAD(P)H dehydrogenase, quinone 2, also known as QR2, is a protein that in humans is encoded by the NQO2 gene. It is a phase II detoxification enzyme which can carry out two or four electron reductions of quinones. Its mechanism of reduction is through a ping-pong mechanism involving its FAD cofactor. Initially in a reductive phase NQO2 binds to reduced dihydronicotinamide riboside (NRH) electron donor, and mediates a hydride transfer from NRH to FAD. Then, in an oxidative phase, NQO2 binds to its quinone substrate and reduces the quinone to a dihydroquinone. Besides the two catalytic FAD, NQO2 also has two zinc ions. It is not clear whether the metal has a catalytic role. NQO2 is a homodimer. NQO2 can be inhibited by resveratrol. One of QR2's binding sites responds to 2-iodomelatonin, and has been referred to as MT3.
Background References
1. Shen, J. et al. 2010. Inactivation of the quinone oxidoreductases NQO1 and NQO2 strongly elevates the incidence and multiplicity of chemically induced skin tumors. Cancer Res. 70: 1006-1014.
2. SHI, z. et al. 2010. The Neuroprotective Effect of Batch-2, an Aqueous Extract From Cat\'s Claw (Uncaria tomentosa) on 6-OHDA-Induced SH-SY5Y Cell Damage. Progress in Biochemistry and Biophysics. 37: 769~778.
Sequence Similarity
Belongs to the NAD(P)H dehydrogenase (quinone) family.
Subcellular Location
Cytoplasm.
Synonyms
DHQV antibody
DIA6 antibody
EC 1.10.99.2 antibody
MGC94180 antibody
NAD(P)H dehydrogenase quinone 2 antibody
NAD(P)H menadione oxidoreductase 1 dioxin inducible 2 antibody
NAD(P)H menadione oxidoreductase 2 dioxin inducible antibody
NMOR2 antibody
NQO 2 antibody
NQO2 antibody
ExpandImages
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Western blot analysis of NQO2 on different lysates with Rabbit anti-NQO2 antibody (ET1610-92) at 1/10,000 dilution.
Lane 1: MCF7 (Human breast cancer cell) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 120s seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1610-92, 1/10,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 26 kDa
Observed band size: 21 kDa -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NQO2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-92, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"