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p21 Mouse Monoclonal Antibody [A8C11]

Usd: 350

Specification

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Catalog# HA601018

p21 Mouse Monoclonal Antibody [A8C11]

Application

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

p21 Mouse Monoclonal Antibody [A8C11]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within full length of human p21.

Species Reactivity

Human

Validated Applications

IHC-P

Molecular Weight

Predicted band size: 18 kDa

Positive Control

Human cervical well-differentiated squamous cell carcinoma tissue, human thyroid carcinoma tissue, human breast carcinoma tissue.

Conjugation

unconjugated

Clone Number

A8C11

RRID

AB_3071742

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:200-1:400

Target

Function

p21WAF1, also known as cyclin-dependent kinase inhibitor 1 or CDK-interacting protein 1, is a protein that in humans is encoded by the CDKN1A gene located on chromosome 6 (6p21.2). May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. p21 is expressed in all adult human tissues. In tumors, the expression of p21 has been studied by immunohistochemical methods in a wide range of human tumors, such as gastric carcinoma, non-small cell lung carcinoma, and thyroid carcinoma. The expression of p21 is associated with favorable prognosis in various tumors.

Background References

1. Kaya Z. et. al. CDKN1A (p21 gene) polymorphisms correlates with age in esophageal cancer. Mol Biol Rep. 2022 Jan

2. Kreis NN. et. al. The Multifaceted p21 (Cip1/Waf1/CDKN1A) in Cell Differentiation, Migration and Cancer Therapy. Cancers (Basel). 2019 Aug

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: P38936 Human

Synonyms

CAP20 antibody

CDK-interacting protein 1 antibody

CDKI antibody

CDKN1 antibody

Cdkn1a antibody

CDN1A_HUMAN antibody

CIP1 antibody

Cyclin Dependent Kinase Inhibitor 1A antibody

Cyclin-dependent kinase inhibitor 1 antibody

Cyclin-dependent kinase inhibitor 1A (P21) antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human cervical well-differentiated squamous cell carcinoma tissue with Mouse anti-p21 antibody (<a href="/products/HA601018" style="font-weight: bold;text-decoration: underline;">HA601018</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601018" style="font-weight: bold;text-decoration: underline;">HA601018</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cervical well-differentiated squamous cell carcinoma tissue with Mouse anti-p21 antibody (HA601018) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601018) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Mouse anti-p21 antibody (<a href="/products/HA601018" style="font-weight: bold;text-decoration: underline;">HA601018</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601018" style="font-weight: bold;text-decoration: underline;">HA601018</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Mouse anti-p21 antibody (HA601018) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601018) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-p21 antibody (<a href="/products/HA601018" style="font-weight: bold;text-decoration: underline;">HA601018</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601018" style="font-weight: bold;text-decoration: underline;">HA601018</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-p21 antibody (HA601018) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601018) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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