p60 CAF1 Recombinant Rabbit Monoclonal Antibody [JG92-30] (ET7108-85)

UNIPROT #

Q13112

PROTEIN NAME

p60 CAF1

SYNONYMS

CAF 1 subunit B antibody;CAF 1A antibody;CAF I 60 kDa subunit antibody;CAF IP60 antibody;CAF-1 antibody;CAF-1 subunit B antibody;CAF-I 60 kDa subunit antibody;CAF-I p60 antibody;CAF1 antibody;CAF1A antibody;CAF1B_HUMAN antibody;CAF1P60 antibody;CHAF 1B antibody;CHAF1B antibody;Chromatin assembly factor 1 subunit B antibody;Chromatin assembly factor I antibody;Chromatin assembly factor I p60 subunit antibody;Human chromatin assembly factor-I p60 subunit antibody;M phase phosphoprotein 7 antibody;M-phase phosphoprotein 7 antibody;MPHOSPH7 antibody;MPP 7 antibody;MPP7 antibody;p60 subunit antibody

SEQUENCE SIMILARITIES

Belongs to the WD repeat HIR1 family.

DEVELOPMENTAL STAGE

Active complex is found in G1, S and G2 phases.

POST-TRANSLATIONAL MODIFICATION

Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.