p75 NGF Receptor Recombinant Rabbit Monoclonal Antibody [SA39-02] (ET1601-22)
Catalog# ET1601-22
Rabbit Monoclonal to p75 NGF Receptor
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
IP
-
FC
-
Human
-
Mouse
-
Rat
Lysates/proteins at 15 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-22) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
p75 NGF Receptor Recombinant Rabbit Monoclonal Antibody [SA39-02] (ET1601-22)
Immunogen
Synthetic peptide within human p75 ngf receptor aa 345-394 / 427.
Host
Rabbit
Positive Control
Neuro-2a cell lysates, PC-12 cell lysates, PC-12-si NT cell lysate, PC-12-si 商品名 cell lysate, Hela, rat uterus tissue, human endometrium tissue, human tonsil tissue, mouse uterus tissue using anti-商品名 antibody. The section was pre-treated using heat mediated antigen retrieval, PC-12, SH-SY5Y cell lysates, Neuro-2a.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SA39-02
RRID
APPLICATION DILUTION
-
WB
-
1:2,000-1:5,000
-
IF-Cell
-
1:50-1:100
-
IF-Tissue
-
1:50-1:100
-
IHC-P
-
1:200-1:1,000
-
IP
-
Use at an assay dependent concentration.
-
FC
-
1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 45 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
p75 NGF Receptor
SYNONYMS
CD271 antibody;CD271 antigen antibody;Gp80 LNGFR antibody;Gp80-LNGFR antibody;Low affinity nerve growth factor receptor antibody;Low affinity neurotrophin receptor p75NTR antibody;Low-affinity nerve growth factor receptor antibody;Nerve growth factor receptor antibody;Nerve growth factor receptor TNFR superfamily member 16 antibody;NGF receptor antibody;Ngfr antibody;p75 ICD antibody;p75 Neurotrophin receptor antibody;p75 NTR antibody;p75(NTR) antibody;p75NTR antibody;TNFR Superfamily Member 16 antibody;TNFRSF16 antibody;TNR16_HUMAN antibody;Tumor necrosis factor receptor superfamily member 16 antibody
POST-TRANSLATIONAL MODIFICATION
N- and O-glycosylated.; O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.; Phosphorylated on serine residues.
SUBCELLULAR LOCATION
Membrane
FUNCTION
The p75 neurotrophin receptor (p75NTR) was first identified in 1973 as the low-affinity nerve growth factor receptor (LNGFR) before discovery that p75NTR bound other neurotrophins equally well as nerve growth factor. p75NTR is a neurotrophic factor receptor. Neurotrophic factor receptors bind Neurotrophins including Nerve growth factor, Neurotrophin-3, Brain-derived neurotrophic factor, and Neurotrophin-4. All neurotrophins bind to p75NTR. This also includes the immature pro-neurotrophin forms. Neurotrophic factor receptors, including p75NTR, are responsible for ensuring a proper density to target ratio of developing neurons, refining broader maps in development into precise connections. p75NTR is involved in pathways that promote neuronal survival and neuronal death.
CITATIONS
-
Yun-jin Zang;Bei Zhang
NLRP3 inflammasome is involved in nerve recovery after sciatic nerve injury
-
WB
-
IHC
-
Mouse
-
Citation
-
-
Shi, C. J., Lian, J. J., Zhang, B. W., Cha, J. X., Hua, Q. H., Pi, X. P., Hou, Y. J., Xie, X., & Zhang, R.
TGFβR-1/ALK5 inhibitor RepSox induces enteric glia-to-neuron transition and influences gastrointestinal mobility in adult mice
-
IF
-
Mouse
-
Citation
-
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.