PAK2 Recombinant Rabbit Monoclonal Antibody [JB41-84] (ET7107-79)

UNIPROT #

Q13177

PROTEIN NAME

PAK2

SYNONYMS

C-t-PAK2 antibody;CB422 antibody;EC 2.7.11.1 antibody;Gamma PAK antibody;Gamma-PAK antibody;hPAK65 antibody;Kinase antibody;p21 (CDKN1A) activated kinase 2 antibody;p21 (CDKN1A)-activated kinase 2a antibody;p21 activated kinase 2 antibody;p21 protein (Cdc42/Rac)-activated kinase 2 antibody;p21 protein Cdc42 Rac activated kinase 2 antibody;p21-activated kinase 2 antibody;p21-activated kinase, 65-KD antibody;p21-activated protein kinase I antibody;p21CDKN1A activated kinase 2 antibody;p27 antibody;p34 antibody;p58 antibody;p65PAK antibody;PAK 2 antibody;PAK-2 antibody;PAK-2p34 antibody;Pak2 antibody;PAK2_HUMAN antibody;PAK65 antibody;PAKgamma antibody;S6 H4 kinase antibody;S6/H4 kinase antibody;Serine threonine protein kinase PAK 2 antibody;Serine/threonine protein kinase PAK 2 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

TISSUE SPECIFICITY

Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.

POST-TRANSLATIONAL MODIFICATION

Full-length PAK2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.; During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.; Ubiquitinated, leading to its proteasomal degradation.; PAK-2p34 is myristoylated.

SUBCELLULAR LOCATION

Cytoplasm, Membrane, Nucleus.

FUNCTION

Three recently identified isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase STE20, involved in pheromone signaling. The α, β, and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.