PDIA6 Recombinant Rabbit Monoclonal Antibody [JF0974] (ET1702-26)
Catalog# ET1702-26
Rabbit Monoclonal to PDIA6
Tested for
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WB
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FC
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IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of PDIA6 on different lysates with Rabbit anti-PDIA6 antibody (ET1702-26) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: K-562 cell lysate
Lane 3: HeLa cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: 4T1 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: Rat liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: Lane 1-3: 25 seconds; Lane 4-7: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: K-562 cell lysate
Lane 3: HeLa cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: 4T1 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: Rat liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: Lane 1-3: 25 seconds; Lane 4-7: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
