Phospho-Creb (S133) Recombinant Rabbit Monoclonal Antibody [JB25-40] (ET7107-93)
Catalog# ET7107-93
Rabbit Monoclonal to Phospho-Creb (S133)
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WB
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IF-Cell
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IHC-P
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IP
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FC
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IF-Tissue
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Human
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Mouse
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Rat
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 25μg/mL anisomycin for 30 minutes whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 40 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-93) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-Creb (S133) Recombinant Rabbit Monoclonal Antibody [JB25-40] (ET7107-93)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser133 of human creb.
Host
Rabbit
Modification
Phospho
Modification Site
S133
Positive Control
HeLa treated with 25μg/mL anisomycin for 30 minutes whole cell lysate, SH-SY5Y, HUVEC, human spleen tissue, mouse colon tissue, human colon carcinoma tissue, human lymph nodes tissue, mouse large intestine tissue, HeLa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JB25-40
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:50-1:100
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IHC-P
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1:50-1:1,000
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
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IF-Tissue:1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 35 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-Creb (S133)
SYNONYMS
Active transcription factor CREB antibody;cAMP response element binding protein 1 antibody;cAMP response element binding protein antibody;cAMP responsive element binding protein 1 antibody;cAMP-responsive element-binding protein 1 antibody;CREB antibody;CREB-1 antibody;CREB1 antibody;CREB1_HUMAN antibody;Cyclic AMP-responsive element-binding protein 1 antibody;MGC9284 antibody;OTTHUMP00000163864 antibody;OTTHUMP00000163865 antibody;OTTHUMP00000206660 antibody;OTTHUMP00000206662 antibody;OTTHUMP00000206667 antibody;Transactivator protein antibody
SEQUENCE SIMILARITIES
Belongs to the bZIP family.
POST-TRANSLATIONAL MODIFICATION
Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. In liver, phosphorylation is induced by fasting or glucagon in a circadian fashion (By similarity). CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity (By similarity). Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1 (By similarity). Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. Phosphorylated by TSSK4 on Ser-133.; Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
CITATIONS
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Pentoxifylline Enhances Antioxidative Capability and Promotes Mitochondrial Biogenesis in D-Galactose-Induced Aging Mice by Increasing Nrf2 and PGC-1α through the cAMP-CREB Pathway
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WB
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Human
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Citation
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JianhaoYang,et al
Exploring the mechanism of action of Huoermai Essential Oil for plateau insomnia based on the cAMP/CREB/BDNF/GABAergic pathway
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WB
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Mouse
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Citation
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Zhang, X., Deng, D., Cui, D., Liu, Y., He, S., Zhang, H., Xie, Y., Yu, X., Yang, S., Chen, Y., & Su, Z.
Cholesterol Sulfate Exerts Protective Effect on Pancreatic β-Cells by Regulating β-Cell Mass and Insulin Secretion
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WB
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Mouse
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Rat
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Citation
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Zhao Meihuan,et al
Gastrodin relieves Parkinson's disease-related motor deficits by facilitating the MEK-dependent VMAT2 to maintain dopamine homeostasis
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WB
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Mouse
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Citation
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-
Zhiguang Su
Regulation of hepatic gluconeogenesis by nuclear factor Y transcription factor in mice
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WB
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Mouse
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Citation
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