Phospho-Cyclin E1 (T77) Recombinant Rabbit Monoclonal Antibody [SD2025] (ET1612-31)

UNIPROT #

P24864

PROTEIN NAME

Phospho-Cyclin E1 (T77)

SYNONYMS

CCNE antibody;Ccne1 antibody;CCNE1_HUMAN antibody;cyclin E variant ex5del antibody;cyclin E variant ex7del antibody;Cyclin E1 antibody;Cyclin Es antibody;Cyclin Et antibody;CyclinE antibody;G1/S specific cyclin E antibody;G1/S-specific cyclin-E1 antibody

SEQUENCE SIMILARITIES

Belongs to the cyclin family. Cyclin E subfamily.

TISSUE SPECIFICITY

Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.; Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.