Phospho-YAP1 (S127) Recombinant Rabbit Monoclonal Antibody [SN0718] (ET1611-69)

UNIPROT #

P46937

PROTEIN NAME

Phospho-YAP1 (S127)

SYNONYMS

65 kDa Yes associated protein antibody;65 kDa Yes-associated protein antibody;COB1 antibody;YAp 1 antibody;YAP 65 antibody;YAP antibody;YAP1 antibody;YAP1_HUMAN antibody;YAP2 antibody;YAP65 antibody;yes -associated protein delta antibody;Yes associated protein 1 65kDa antibody;Yes associated protein 1 antibody;Yes associated protein 2 antibody;yes associated protein beta antibody;YKI antibody;Yorkie homolog antibody

SEQUENCE SIMILARITIES

Belongs to the YAP1 family.

TISSUE SPECIFICITY

Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage. Phosphorylation at Ser-400 and Ser-403 by CK1 is triggered by previous phosphorylation at Ser-397 by LATS proteins and leads to YAP1 ubiquitination by SCF(beta-TRCP) E3 ubiquitin ligase and subsequent degradation. Phosphorylated at Thr-119, Ser-138, Thr-154, Ser-367 and Thr-412 by MAPK8/JNK1 and MAPK9/JNK2, which is required for the regulation of apoptosis by YAP1.; Ubiquitinated by SCF(beta-TRCP) E3 ubiquitin ligase.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

The Yes-associated protein, otherwise known as YAP, is a 14-3-3-binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ-binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis.