PIM1 Recombinant Rabbit Monoclonal Antibody [ST0513] (ET1609-57)
Catalog# ET1609-57
Rabbit Monoclonal to PIM1
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
Lane 1: K-562 cell lysate
Lane 2: TF-1 cell lysate
Lane 3: 22RV1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-57) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PIM1 Recombinant Rabbit Monoclonal Antibody [ST0513] (ET1609-57)
Immunogen
Synthetic peptide within human pim1 aa 20-60.
Host
Rabbit
Positive Control
K-562 cell lysate, TF-1 cell lysate, 22RV1 cell lysate, K-562, BT-20, HepG2, SW480, human tonsil tissue, human colon carcinoma tissue, human spleen tissue, human breast carcinoma tissue, human breast tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
ST0513
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:400
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IHC-P
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1:50-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 36 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PIM1
SYNONYMS
Oncogene PIM 1 antibody;Oncogene PIM1 antibody;PIM 1 antibody;pim 1 kinase 44 kDa isoform antibody;Pim 1 kinase antibody;pim 1 oncogene (proviral integration site 1) antibody;Pim 1 oncogene antibody;PIM antibody;PIM1 antibody;pim1 kinase 44 kDa isoform antibody;PIM1_HUMAN antibody;Pim2 antibody;PIM3 antibody;Proto oncogene serine/threonine protein kinase Pim 1 antibody;Proto-oncogene serine/threonine-protein kinase Pim-1 antibody;Proviral integration site 1 antibody;Proviral integration site 2 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
TISSUE SPECIFICITY
Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
POST-TRANSLATIONAL MODIFICATION
Autophosphorylated on both serine/threonine and tyrosine residues. Phosphorylated. Interaction with PPP2CA promotes dephosphorylation.; Ubiquitinated, leading to proteasomal degradation.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Cell membrane.
FUNCTION
Pim-1 is a serine/threonine kinase that cooperates with c-Myc in lymphoid cell transformation. The expression of pim-1 increases during the progression from early to late G1, remaining high at the G1/S boundary and G2 phases of the cell cycle. Pim-1 is regulated at both the transcriptional and translational level, and it has been shown to be induced by IL-2 stimulation. Pim-1 also plays a role in T cell differentiation, and it has been shown to stimulate c-Myc-mediated apoptosis upstream of caspase-3-like proteases.
CITATIONS
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Wen, Q. L., Yi, H. Q., Yang, K., Yin, C. T., Yin, W. J., Xiang, F. Y., Bao, M., Shuai, J., Song, Y. W., Ge, M. H., & Zhu, X.
Role of oncogene PIM-1 in the development and progression of papillary thyroid carcinoma: Involvement of oxidative stress. Molecular and cellular endocrinology, 523, 111144.
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WB
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Human
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Citation
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Targeting macrophagic PIM-1 alleviates osteoarthritis by inhibiting NLRP3 inflammasome activation via suppressing mitochondrial ROS/Cl- efflux signaling pathway
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WB
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Human
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Citation
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Xu Qianqian,et al
Combined BRAF and PIM1 inhibitory therapy for papillary thyroid carcinoma based on BRAFV600E regulation of PIM1: Synergistic effect and metabolic mechanisms
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IHC
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WB
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Mouse
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Citation
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