Placental alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [JM22-53] (ET1705-21)
Catalog# ET1705-21
Rabbit Monoclonal to Placental alkaline phosphatase
Tested for
-
WB
-
IF-Tissue
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Placental alkaline phosphatase on different lysates with Rabbit anti-Placental alkaline phosphatase antibody (ET1705-21) at 1/5,000 dilution.
Lane 1: A431 cell lysate
Lane 2: SiHa cell lysate
Lane 3: AGS cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 70 kDa
Exposure time: 1 minute 14 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-21) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate
Lane 2: SiHa cell lysate
Lane 3: AGS cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 70 kDa
Exposure time: 1 minute 14 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-21) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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