Rat IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH02-60] - BSA and Azide free (Detector)
Overview
Product Name
Rat IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH02-60] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Rat IL-1 beta aa 117-268 (Q63264).
Species Reactivity
Rat
Validated Applications
ELISA(Det)
Molecular Weight
Predicted band size: 30.6 kDa
Positive Control
Recombinant Rat il-1 beta protein (HA210708).
Conjugation
unconjugated
Clone Number
PSH02-60
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-59] to Rat IL-1 beta (Capture) (HA721836) and recombinant standard Rat IL-1 beta (HA210708) as the standard. The reference range value is 30.9-2,500 pg/mL.
Target
Function
Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. IL1B production occurs in 2 steps, each being controlled by different stimuli. First, inflammatory signals, such as LPS, stimulate the synthesis and promote the accumulation of cytosolic stores of pro-IL1B (priming). Then additional signals are required for inflammasome assembly, leading to CASP1 activation, pro-IL1B processing and eventually secretion of the active cytokine. IL1B processing and secretion are temporarily associated.
Background References
1. Qiao J et al. Autologous platelet rich plasma injection can be effective in the management of osteoarthritis of the knee: impact on IL-1 beta, TNF-alpha, hs-CRP. J Orthop Surg Res. 2024 Oct
2. Vicens-Artés S et al. Effect of MP-AzeFlu in IL-1 beta-induced IL-6 and proinflammatory cytokines. Immunol Res. 2023 Jun
Subcellular Location
Secreted.
UNIPROT
Synonyms
Catabolin antibody
H1 antibody
IFN beta inducing factor antibody
IL 1 antibody
IL 1 beta antibody
IL-1 beta antibody
IL1 antibody
IL1 BETA antibody
IL1B antibody
IL1B_HUMAN antibody
ExpandImages
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![Sandwich ELISA analysis of rat IL1β matched pair antibodies<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721836" style="font-weight: bold;text-decoration: underline;">HA721836</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted rat IL1β protein (<a href="/products/HA210708" style="font-weight: bold;text-decoration: underline;">HA210708</a>) starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH02-60]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking separately in sequence. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721837_1.jpg?v=20260509231241)
Sandwich ELISA analysis of rat IL1β matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721836) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted rat IL1β protein (HA210708) starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH02-60]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking separately in sequence. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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