RYR1 Rabbit Polyclonal Antibody
Catalog# ER1803-19
RYR1 Rabbit Polyclonal Antibody
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Dot Blot
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IHC-P
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
RYR1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human RYR1 aa 1,354-1,403 / 5,038.
Species Reactivity
Human, Mouse
Validated Applications
Dot Blot, IHC-P, FC
Molecular Weight
Predicted band size: 565 kDa
Positive Control
Human fetal skeletal muscle tissue, mouse cerebellum tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, SiHa.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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Dot Blot
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1:500-1:1,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
Calcium channel that mediates the release of Ca2+ from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca2+ leaking into the cytoplasm. Can also mediate the release of Ca2+ from intracellular stores in neurons, and may thereby promote prolonged Ca2+ signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis.
Background References
1. Tilgen N et al. Identification of four novel mutations in the C-terminal membrane spanning domain of the ryanodine receptor 1: association with central core disease and alteration of calcium homeostasis. Hum Mol Genet 10:2879-2887 (2001).
2. Monnier N et al. Correlations between genotype and pharmacological, histological, functional, and clinical phenotypes in malignant hyperthermia susceptibility. Hum Mutat 26:413-425 (2005).
Sequence Similarity
Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR1 subfamily.
Tissue Specificity
Skeletal muscle and brain (cerebellum and hippocampus).
Post-translational Modification
Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2843 may increase channel activity. Repeated very high-level exercise increases phosphorylation at Ser-2843.; Activated by reversible S-nitrosylation (By similarity). Repeated very high-level exercise increases S-nitrosylation.
Subcellular Location
Sarcoplasmic reticulum membrane.
Synonyms
Arrhythmogenic right ventricular dysplasia 2 antibody
ARVC2 antibody
ARVD2 antibody
Brain ryanodine receptor calcium release channel antibody
Brain type ryanodine receptor antibody
Cardiac muscle ryanodine receptor calcium release channel antibody
Cardiac muscle type ryanodine receptor antibody
CCO antibody
Central core disease of muscle antibody
HBRR antibody
ExpandImages
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Dot blot analysis of anti-RYR1 immunization peptide on PVDF. 1ug, 2ug and 4ug peptides were given in this test. Anti-RYR1 antibody was diluted with 1/500.
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Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-RYR1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-RYR1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-RYR1 antibody (ER1803-19) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-19) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-RYR1 antibody (ER1803-19) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-19) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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