Smad1 Recombinant Rabbit Monoclonal Antibody [SY0254] (ET1607-42)
Catalog# ET1607-42
Rabbit Monoclonal to Smad1
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
All lanes: Western blot analysis of Smad1 with anti-Smad1 antibody (ET1607-42) at 1:500 dilution.
Lane 1: Wild-type p19 whole cell lysate (15 µg).
Lane 2/3: Smad1 knockout p19 whole cell lysate (15 µg).
ET1607-42 was shown to specifically react with Smad1 in wild-type p19 cells. No bands were observed when Smad1 knockout samples were tested. Wild-type and Smad1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1607-42, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:10,000 dilution was used for 1 hour at room temperature.
Lane 1: Wild-type p19 whole cell lysate (15 µg).
Lane 2/3: Smad1 knockout p19 whole cell lysate (15 µg).
ET1607-42 was shown to specifically react with Smad1 in wild-type p19 cells. No bands were observed when Smad1 knockout samples were tested. Wild-type and Smad1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1607-42, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:10,000 dilution was used for 1 hour at room temperature.