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SOX9 Recombinant Rabbit Monoclonal Antibody [SN74-09] (ET1611-56)

Price: Regular price $385.00

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Catalog# ET1611-56

Rabbit Monoclonal to SOX9

Tested for

  • WB

  • IF-Cell

  • IHC-P

  • IP

  • FC

  • CUT&Tag-seq

  • mIHC

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

SOX9 Recombinant Rabbit Monoclonal Antibody [SN74-09] (ET1611-56)

Immunogen

Recombinant protein within human sox9 aa 140-340.

Host

Rabbit

Positive Control

SW480 cell lysate, MDA-MB-231 cell lysate, PANC-1 cell lysate, NIH/3T3 cell lysate, MDA-MB-231, human tonsil tissue, human breast carcinoma tissue, mouse liver tissue, human colon tissue, human thyroid tissue, human breast tissue, human small intestine tissue, human prostate carcinoma tissue, human stomach carcinoma tissue, human lung carcinoma tissue, rat colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN74-09

RRID

AB_2924312

APPLICATION DILUTION

  • WB

  • 1:10,000

  • IF-Cell

  • 1:500

  • IHC-P

  • 1:50-1:1,000

  • FC

  • 1:2,000

  • IP

  • Use at an assay dependent concentration.

  • mIHC

  • 1:1,500

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 56 kDa

Isotype

IgG

TARGET

UNIPROT #

P48436

PROTEIN NAME

SOX9

SYNONYMS

campomelic dysplasia autosomal sex reversal antibody;CMD 1 antibody;CMD1 antibody;CMPD 1 antibody;CMPD1 antibody;SOX 9 antibody;Sox9 antibody;SOX9_HUMAN antibody;SRA 1 antibody;SRA1 antibody;SRXX2 antibody;SRXY10 antibody;SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody;SRY (sex determining region Y) box 9 antibody;SRY (sex determining region Y)-box 9 antibody;SRY (sex-determining region Y)-box 9 protein antibody;SRY related HMG box gene 9 antibody;Transcription factor SOX 9 antibody;Transcription factor SOX-9 antibody;transcription factor SOX9 antibody

POST-TRANSLATIONAL MODIFICATION

Acetylated; acetylation impairs nuclear localization and ability to transactivate expression of target genes. Deacetylated by SIRT1.; Phosphorylation at Ser-64 and Ser-211 by PKA increases transcriptional activity and may help delay chondrocyte maturation downstream of PTHLH/PTHrP signaling. Phosphorylation at either Ser-64 or Ser-211 is required for sumoylation, but phosphorylation is not dependent on sumoylation. Phosphorylated on tyrosine residues; tyrosine dephosphorylation by PTPN11/SHP2 blocks SOX9 phosphorylation by PKA and subsequent SUMOylation.; Ubiquitinated; ubiquitination leads to proteasomal degradation and is negatively regulated by DDRGK1.; Sumoylated; phosphorylation at either Ser-64 or Ser-211 is required for sumoylation. Sumoylation is induced by BMP signaling pathway.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

SOX-9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It is expressed by proliferating but not hypertrophic chondrocytes that is essential for differentiation of precursor cells into chondrocytes and, with steroidogenic factor 1, regulates transcription of the anti-Müllerian hormone (AMH) gene. SOX-9 also plays a pivotal role in male sexual development; by working with Sf1, SOX-9 can produce AMH in Sertoli cells to inhibit the creation of a female reproductive system. It also interacts with a few other genes to promote the development of male sexual organs. The process starts when the transcription factor Testis determining factor (encoded by the sex-determining region SRY of the Y chromosome) activates SOX-9 activity by binding to an enhancer sequence upstream of the gene. Next, Sox9 activates FGF9 and forms feedforward loops with FGF9[ and PGD2. These loops are important for producing SOX-9; without these loops, SOX-9 would run out and the development of a female would almost certainly ensue. Activation of FGF9 by SOX-9 starts vital processes in male development, such as the creation of testis cords and the multiplication of Sertoli cells.

CITATIONS

  • Jian Chen;Fengdong Zhao

    The RCAN1.4-calcineurin/NFAT signaling pathway is essential for hypoxic adaption of intervertebral discs

  • Ji Zhongyin,et al

    Injectable hydrogel encapsulating siMMP13 with anti-ROS and anti-apoptotic functions for osteoarthritis treatment

  • Che Feng,et al

    Noninvasive identification of SOX9 status using radiomics signatures may help construct personalized treatment strategy in hepatocellular carcinoma

  • Zheng X, Qiu J, Gao N, et al

    Paroxetine Attenuates Chondrocyte Pyroptosis and Inhibits Osteoclast Formation by Inhibiting NF-κB Pathway Activation to Delay Osteoarthritis Progression

  • Zhao, C., Matalonga, J., Lancman, J. J., Liu, L., Xiao, C., Kumar, S., Gates, K. P., He, J., Graves, A., Huisken, J., Azuma, M., Lu, Z., Chen, C., Ding, B. S., & Dong, P. D. S.

    Regenerative failure of intrahepatic biliary cells in Alagille syndrome rescued by elevated Jagged/Notch/Sox9 signaling

  • Liao Yuansong,et al

    MSX1+ PDGFRAlow limb mesenchyme-like cells as an efficient stem cell source for human cartilage regeneration

  • Zheng, X., Qiu, J., Pan, W., Gong, Y., Zhang, W., Jiang, T., Chen, L., Chen, W., & Hong, Z.

    Selumetinib - a potential small molecule inhibitor for osteoarthritis treatment

  • Sun, Q., Tian, F. M., Liu, F., Fang, J. K., Hu, Y. P., Lian, Q. Q., Zhou, Z., & Zhang, L.

    Denosumab alleviates intervertebral disc degeneration adjacent to lumbar fusion by inhibiting endplate osteochondral remodeling and vertebral osteoporosis in ovariectomized rats. Arthritis research & therapy, 23(1), 152.

  • Xiao Juan,et al

    Icariin inhibits chondrocyte ferroptosis and alleviates osteoarthritis by enhancing the SLC7A11/GPX4 signaling

  • Zhang Lina,et al

    YAP maintains cartilage stem/progenitor cell homeostasis in osteoarthritis

  • Ji Zhongyin,et al

    Mrgprb2-mediated mast cell activation exacerbates Modic changes by regulating immune niches

  • Yi Zhang,et al

    An Optimized Protocol for Multiple Immunohistochemical Staining of Fragile Tissue Samples

  • Shu, Y., Xu, Q., Xu, Y., Tao, Q., Shao, M., Cao, X., Chen, Y., Wu, Z., Chen, M., Zhou, Y., Zhou, P., Shi, Y., & Bu, H.

    Loss of Numb promotes hepatic progenitor expansion and intrahepatic cholangiocarcinoma by enhancing Notch signaling

  • Sun Peihao,et al

    Maternal exposure to DON during lactation induces testicular toxicity in pubertal and adult offspring mice

  • Liao Taiyang,et al

    Total glucosides of white paeony capsule alleviate articular cartilage degeneration and aberrant subchondral bone remodeling in knee osteoarthritis

  • Activation of Nrf2 signaling by 4-octyl itaconate attenuates the cartilaginous endplate degeneration by inhibiting E3 ubiquitin ligase ZNF598

  • Song Kangping,et al

    Pulsed electromagnetic fields potentiate bone marrow mesenchymal stem cell chondrogenesis by regulating the Wnt/β-catenin signaling pathway

  • Hu, H., Duan, Y., Wang, K., Fu, H., Liao, Y., Wang, T., Zhang, Z., Kang, F., Zhang, B., Zhang, H., Huo, F., Yin, Y., Chen, G., Hu, H., Cai, H., Tian, W., & Li, Z.

    Dental niche cells directly contribute to tooth reconstitution and morphogenesis

  • Deng Junjie,et al

    Biomimetic zwitterionic copolymerized chitosan as an articular lubricant

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