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Taq DNA Polymerase Mouse Monoclonal Antibody [3C3] (EM1701-49)

Price: Regular price $350.00

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Catalog# EM1701-49

Mouse Monoclonal to Taq DNA Polymerase

Tested for

  • Antibody-mediated hot start PCR

Specifications

Product Type

Mouse monoclonal primary

Product Name

Taq DNA Polymerase Mouse Monoclonal Antibody [3C3] (EM1701-49)

Immunogen

Taq dna polymerase

Host

Mouse

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

3C3

RRID

AB_3068773

APPLICATION DILUTION

  • Hot start PCR

  • 1:1,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze/thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2ug/ul

Purification

Protein G affinity purified.

Isotype

IgG1

TARGET

PROTEIN NAME

Taq DNA Polymerase

FUNCTION

Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. Anti-Taq Antibody is an ideal tool for hot-start PCR with Taq DNA polymerase. The Anti-Taq Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing non-specific and primer dimer amplification resulted from non-specific priming at ambient temperature for the duration of time prior to PCR thermal cycling. During the initial denaturing step in PCR thermal cycling, the Anti-Taq Antibody is denatured and the Taq DNA polymerase is then released, thus regaining its full DNA polymerase activity. The result indicates that anti-Taq DNA Polymerase antibody increases the specificity and sensitivity of the PCR.

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