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TMS1 / ASC Recombinant Rabbit Monoclonal Antibody [PSH17-35] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751623

TMS1 / ASC Recombinant Rabbit Monoclonal Antibody [PSH17-35] - BSA and Azide free

Application

  • WB

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

TMS1 / ASC Recombinant Rabbit Monoclonal Antibody [PSH17-35] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse TMS1 / ASC aa 1-193.

Species Reactivity

Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 21 kDa

Positive Control

Mouse spleen tissue lysate, Mouse colon tissue lysate, Rat spleen tissue lysate, Rat colon tissue lysate.

Conjugation

unconjugated

Clone Number

PSH17-35

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. It is localized mainly in the nucleus of monocytes and macrophages. In case of pathogen infection, however, it relocalizes rapidly to the cytoplasm, perinuclear space, endoplasmic reticulum and mitochondria and it is a key adaptor protein in activation of the inflammasome.

Background References

1. Jiang H et al. Pristimerin suppresses AIM2 inflammasome by modulating AIM2-PYCARD/ASC stability via selective autophagy to alleviate tendinopathy. Autophagy. 2024 Jan

2. Suganuma Y et al. Expression of a PYCARD/ASC variant lacking exon 2 in Japanese patients with palindromic rheumatism increases interleukin-1beta secretion. Asian Pac J Allergy Immunol. 2022 Mar

Subcellular Location

Cytoplasm, Inflammasome, Endoplasmic reticulum, Mitochondrion, Nucleus.

UNIPROT

SwissProt: Q9EPB4 Mouse

Entrez Gene: 282817 Rat

Synonyms

Apoptosis associated speck like protein containing a CARD antibody

Apoptosis-associated speck-like protein containing a CARD antibody

ASC antibody

CARD 5 antibody

CARD5 antibody

Caspase recruitment domain containing protein 5 antibody

Caspase recruitment domain protein 5 antibody

Caspase recruitment domain-containing protein 5 antibody

hASC antibody

MGC10332 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody (<a href="/products/HA751623" style="font-weight: bold;text-decoration: underline;">HA751623</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse spleen tissue lysate<br />Lane 2: Mouse colon tissue lysate<br />Lane 3: Mouse skeletal muscle tissue lysate (low expression)<br />Lane 4: Rat spleen tissue lysate<br />Lane 5: Rat colon tissue lysate<br />Lane 6: Rat skeletal muscle tissue lysate (low expression)<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751623" style="font-weight: bold;text-decoration: underline;">HA751623</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody (HA751623) at 1/5,000 dilution.

    Lane 1: Mouse spleen tissue lysate
    Lane 2: Mouse colon tissue lysate
    Lane 3: Mouse skeletal muscle tissue lysate (low expression)
    Lane 4: Rat spleen tissue lysate
    Lane 5: Rat colon tissue lysate
    Lane 6: Rat skeletal muscle tissue lysate (low expression)

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751623) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"