TXNIP Recombinant Rabbit Monoclonal Antibody [JM60-35] (ET1705-72)
Catalog# ET1705-72
Rabbit Monoclonal to TXNIP
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: Mouse stomach tissue lysate
Lane 5: Mouse kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 50 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-72) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
TXNIP Recombinant Rabbit Monoclonal Antibody [JM60-35] (ET1705-72)
Immunogen
Synthetic peptide within human txnip aa 50-99 / 391.
Host
Rabbit
Positive Control
HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse stomach tissue lysate, mouse kidney tissue lysate, PC-12, human kidney tissue, mouse kidney tissue, rat kidney tissue, human stomach tissue, mouse esophagus tissue, mouse stomach tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM60-35
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:200-1:500
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IHC-P
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1:400-1:5,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 44 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
TXNIP
SYNONYMS
EST01027 antibody;HHCPA78 antibody;THIF antibody;Thioredoxin binding protein 2 antibody;Thioredoxin interacting protein antibody;Thioredoxin-binding protein 2 antibody;Thioredoxin-interacting protein antibody;TXNIP antibody;TXNIP_HUMAN antibody;Upregulated by 1,25 dihydroxyvitamin D 3 antibody;VDUP1 antibody;Vitamin D3 up-regulated protein 1 antibody
SEQUENCE SIMILARITIES
Belongs to the arrestin family.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells.
CITATIONS
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Zhang, X., Zhao, S., Yuan, Q., Zhu, L., Li, F., Wang, H., Kong, D., & Hao, J.
TXNIP, a novel key factor to cause Schwann cell dysfunction in diabetic peripheral neuropathy, under the regulation of PI3K/Akt pathway inhibition-induced DNMT1 and DNMT3a overexpression. Cell death & disease, 12(7), 642.
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WB
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IHC
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IF
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Citation
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Yanping, D., Xiaoqing, D., Yifan, M. A., Lili, C., Jie, Z., Xinyan, L. I., & Baoping, S.
Astragaloside IV plays a role in reducing radiation-induced liver inflammation in mice by inhibiting thioredoxin-interacting protein/nod-like receptor protein 3 signaling pathway
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WB
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Mouse
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Citation
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Cao Xiang,et al
MicroRNA-204-5p attenuates oxidative stress, apoptosis and inflammation by targeting TXNIP in diabetic cataract
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WB
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Human
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Citation
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Wang Yue,et al
Artemisinin attenuated ischemic stroke induced pyroptosis by inhibiting ROS/TXNIP/NLRP3/Caspase-1 signaling pathway
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WB
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Rat
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Citation
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Chen Qingqiu,et al
REDD1 knockdown ameliorates endothelial cell senescence through repressing TXNIP-mediated oxidative stress
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WB
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Human
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Citation
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Wu Wentong,et al
Rutin attenuates ensartinib-induced hepatotoxicity by non-transcriptional regulation of TXNIP
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IF
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IHC
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Mouse
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Citation
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