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Vimentin Recombinant Rabbit Monoclonal Antibody [SC60-05] (ET1610-39)

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Catalog# ET1610-39

Rabbit Monoclonal to Vimentin

Tested for

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

  • FC

  • IHC-Fr

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Vimentin Recombinant Rabbit Monoclonal Antibody [SC60-05] (ET1610-39)

Immunogen

Synthetic peptide within c-terminal human vimentin.

Host

Rabbit

Positive Control

HeLa cell lysate, HEK-293 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, C2C12 cell lysate, RAW264.7 cell lysate, C6, HeLa, L6, C2C12, human endometrial carcinoma tissue, mouse colon tissue, mouse cerebellum tissue, human breast carcinoma tissue, human colon tissue, human kidney tissue, mouse large intestine tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC60-05

RRID

AB_3069923

APPLICATION DILUTION

  • WB

  • 1:20,000

  • IF-Cell

  • 1:100-1:500

  • IF-Tissue

  • 1:1,000

  • IHC-P

  • 1:1,000-1:10,000

  • IHC-Fr

  • 1:50-1:1,000

  • FC

  • 1:1,000

  • IP

  • Use at an assay dependent concentration.

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 54 kDa

Isotype

IgG

TARGET

UNIPROT #

P08670

PROTEIN NAME

Vimentin

SYNONYMS

CTRCT30 antibody;Epididymis luminal protein 113 antibody;FLJ36605 antibody;HEL113 antibody;VIM antibody;VIME_HUMAN antibody;Vimentin antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

POST-TRANSLATIONAL MODIFICATION

Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS.; O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.; S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types: fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated or sarcomatoid carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein): Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or a malignant melanoma. However, in biopsies representing only a sarcomatoid part of renal cell carcinoma a.o. a strong positivity for vimentin without cytokeratin expression may be seen. Tumours like lymphomas and seminomas have the same intermediate filament profile, but the vimentin expression is usually weaker.

CITATIONS

  • Junjiang Liu,et al

    TAM‐Derived Exosomes Promote EMT by Upregulating lncRNA MIR4435‐2HG in Head and Neck Cancer

  • Yang Yanfang,et al

    SUMOylation of annexin A6 retards cell migration and tumor growth by suppressing RHOU/AKT1-involved EMT in hepatocellular carcinoma

  • Hyaluronic acid mediated Fe3O4 nanocubes reversing the EMT through targeted cancer stem cell

  • Qian Yiming,et al

    Deguelin inhibits the glioblastoma progression through suppressing CCL2/NFκB signaling pathway

  • Fangquan Wu ,et al

    Reduced Proline-Rich Tyrosine Kinase 2 Promotes Tumor Metastasis by Activating Epithelial–Mesenchymal Transition in Colorectal Cancer

  • Wu Wensi,et al

    circGRHPR inhibits aberrant epithelial-mesenchymal transformation progression of lung epithelial cells associated with idiopathic pulmonary fibrosis

  • Zhang Wei,et al

    Columbianadin suppresses glioblastoma progression by inhibiting the PI3K-Akt signaling pathway

  • Mu Mingshan,et al

    FAM188B promotes the growth, metastasis, and invasion of hepatocellular carcinoma by targeting the HRNPA1/PKM2 axis

  • Ginsenoside Rg3 suppresses epithelial-mesenchymal transition via downregulating notch-hes1 signaling in colon cancer cells

  • Jiang Shuanghong,et al

    ARHGAP4 promotes colon cancer metastasis through the TGF-β signaling pathway and may be associated with T cell exhaustion

  • Liu, Y., Hao, Y., Zhao, H., Zhang, Y., Cheng, D., Zhao, L., Peng, Y., Lu, Y., & Li, Y.

    PlexinA1 activation induced by β2-AR promotes epithelial-mesenchymal transition through JAK-STAT3 signaling in human gastric cancer cells

  • Lu Shuanghui,et al

    Downregulation of PDZK1 by TGF-β1 promotes renal fibrosis via inducing epithelial-mesenchymal transition of renal tubular cells

  • Pan Zhengyang,et al

    Galectin-1 Promotes Gastric Carcinoma Progression and Cisplatin Resistance Through the NRP-1/c-JUN/Wee1 Pathway

  • Liu Qingyang,et al

    Sinomenine Alleviates Rheumatoid Arthritis by Suppressing the PI3K-Akt Signaling Pathway, as Demonstrated Through Network Pharmacology, Molecular Docking, and Experimental Validation

  • Minglu Zhou,et al

    Cell surface patching via CXCR4-targeted nanothreads for cancer metastasis inhibition

  • Zhao Q, He W, Liu Z, et al

    LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX

  • Bai Hongxia,et al

    MiRNA-145-5p inhibits gastric cancer progression via the serpin family E member 1-extracellular signal-regulated kinase-1/2 axis

  • Du Hong,et al

    Super-enhancer-associated LINC00963 promotes epithelial-mesenchymal transition in gastric cancer

  • Jiang Wei,et al

    miR-520f-3p blocks MNNG-induced gastric precancerous lesions via the KLF7/NFκB pathway

  • Luo, C., Zhou, M., Chen, C., Li, S., Li, Q., Huang, Y., & Zhou, Z.

    A liposome-based combination strategy using doxorubicin and a PI3K inhibitor efficiently inhibits pre-metastatic initiation by acting on both tumor cells and tumor-associated macrophages

  • Fang T, Jiang J, Yu W, Li R, Tian H

    DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c-Myc signaling pathway

  • Interleukin-6 and Hypoxia Synergistically Promote EMT-Mediated Invasion in Epithelial Ovarian Cancer via the IL-6/STAT3/HIF-1 α Feedback Loop

  • LLGL2 Increases Ca2+ Influx and Exerts Oncogenic Activities via PI3K/AKT Signaling Pathway in Hepatocellular Carcinoma

  • Zhou Heng,et al

    Modulation of epithelial-mesenchymal transition by gemcitabine: Targeting ionizing radiation-induced cellular senescence in lung cancer cell

  • Li Jingyi,et al

    2-Dodecyl-6-Methoxycyclohexa-2, 5-Diene-1, 4-Dione isolated from Averrhoa carambola L. root inhibits high glucose-induced EMT in HK-2 cells through targeting the regulation of miR-21-5p/Smad7 signaling pathway

  • Xu, F., Zhang, J., Ji, L., Cui, W., Cui, J., Tang, Z., Sun, N., Zhang, G., Guo, M., Liu, B., & Dong, J.

    Inhibition of Non-small Cell Lung Cancer by Ferroptosis and Apoptosis Induction through P53 and GSK-3β/Nrf2 Signal Pathways using Qingrehuoxue Formula

  • Xi Xinran,et al

    Identification of PRODH as a mitochondria- and angiogenesis-related biomarker for lung adenocarcinoma

  • Tang Yong,et al

    OLFM2 promotes epithelial-mesenchymal transition, migration, and invasion in colorectal cancer through the TGF-β/Smad signaling pathway

  • Wang Jinqing,et al

    IGFBP7 promotes gastric cancer by facilitating epithelial-mesenchymal transition of gastric cells

  • Acute myeloid leukemia (AML)‐derived mesenchymal stem cells induce chemoresistance and epithelial–mesenchymal transition‐like program in AML through IL‐6/JAK2/STAT3 signaling

  • Ruixi Luo, Yaqiong Wei, Peng Chen, Jing Zhang, La Wang, Wenjia Wang, Ping Wang, Weiyi Tian

    Mesenchymal Stem Cells Inhibit Epithelial-to-Mesenchymal Transition by Modulating the IRE1α Branch of the Endoplasmic Reticulum Stress Response

  • Retinal pigment epithelial cells secrete miR-202-5p-containing exosomes to protect against proliferative diabetic retinopathy

  • Hu, J., Cai, D., Zhao, Z., Zhong, G. C., & Gong, J.

    Suppression of Heterogeneous Nuclear Ribonucleoprotein C Inhibit Hepatocellular Carcinoma Proliferation, Migration, and Invasion via Ras/MAPK Signaling Pathway. Frontiers in oncology, 11, 659676.

  • Function of hsa_circ_0006646 as a competing endogenous RNA to promote progression in gastric cancer by regulating the miR-665-HMGB1 axis

  • Li, S., Li, L., Lin, X., Chen, C., Luo, C., & Huang, Y.

    Targeted Inhibition of Tumor Inflammation and Tumor-Platelet Crosstalk by Nanoparticle-Mediated Drug Delivery Mitigates Cancer Metastasis

  • Yanfang Bai,et al

    Tanshinone I improves renal fibrosis by promoting gluconeogenesis through upregulation of peroxisome proliferator-activated receptor-γ coactivator 1α

  • Hu, Y., Liu, H., Zhu, Z., Qi, X., Yuan, W., Tian, M., Wang, D., & Xu, J.

    LACTB suppresses migration and invasion of glioblastoma via downregulating RHOC/Cofilin signaling pathway

  • Li, Q., Hong, Y., Chen, J., Zhou, X., Tian, X., Yu, Y., Shen, L., Long, C., Cai, M., Wu, S., & Wei, G.

    Hypoxia-Induced HIF-1α Expression Promotes Neurogenic Bladder Fibrosis via EMT and Pyroptosis

  • Zhang Zizhen,et al

    Influential upregulation of KCNE4: Propelling cancer associated fibroblasts-driven colorectal cancer progression

  • Shi Yaxin,et al

    Paris polyphylla ethanol extract and polyphyllin I ameliorate adenomyosis by inhibiting epithelial–mesenchymal transition

  • Heng Zhou,et al

    The role of apoptosis, immunogenic cell death, and macrophage polarization in carbon ion radiotherapy for keloids: Targeting the TGF-β1/SMADs signaling pathway

  • Zhang Dong,et al

    TNFSF11/TNFRSF11A Axis Amplifies HDM-Induced Airway Remodeling by Strengthening TGFβ1/STAT3 Action

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