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Histone H3 Mouse Monoclonal Antibody [3-C4] (EM30605)

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Catalog# EM30605

Mouse Monoclonal to Histone H3

Tested for

  • WB

  • IHC-P

  • mIHC

  • IF-Tissue

  • ChIP

Reacts with

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

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Specifications

Product Type

Mouse monoclonal primary

Product Name

Histone H3 Mouse Monoclonal Antibody [3-C4] (EM30605)

Immunogen

Synthetic peptide within c-terminal residues of human histone h3.

Host

Mouse

Positive Control

HeLa cell lysate, A549 cell lysate, HT-29 cell lysate, HEK-293 cell lysate, C2C12 cell lysate, L-929 cell lysate, C6 cell lysate, mouse kidney, human liver tissue, human testis tissue, mouse testis tissue, rat testis tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

3-C4

RRID

AB_2890027

APPLICATION DILUTION

  • WB

  • 1:10,000

  • IHC-P

  • 1:1,000-1:2,000

  • mIHC

  • 1:500

  • IF-Tissue

  • 1:200-1:400

  • ChIP

  • Use 0.5~2 μg for 25 μg of chromatin.

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 15 kDa

Isotype

IgG1

TARGET

UNIPROT #

P68431

PROTEIN NAME

Histone H3

SYNONYMS

H3 histone family, member A antibody;H3/A antibody;H31_HUMAN antibody;H3FA antibody;Hist1h3a antibody;HIST1H3B antibody;HIST1H3C antibody;HIST1H3D antibody;HIST1H3E antibody;HIST1H3F antibody;HIST1H3G antibody;HIST1H3H antibody;HIST1H3I antibody;HIST1H3J antibody;histone 1, H3a antibody;Histone cluster 1, H3a antibody;Histone H3.1 antibody;Histone H3/a antibody;Histone H3/b antibody;Histone H3/c antibody;Histone H3/d antibody;Histone H3/f antibody;Histone H3/h antibody;Histone H3/i antibody;Histone H3/j antibody;Histone H3/k antibody;Histone H3/l antibody

SEQUENCE SIMILARITIES

Belongs to the histone H3 family.

DEVELOPMENTAL STAGE

Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

POST-TRANSLATIONAL MODIFICATION

Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.; Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.; Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.; Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication.; Phosphorylated at Thr-4 (H3T3ph) by HASPIN during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Thr-12 (H3T11ph) by chromatin-associated CHEK1 regulates the transcription of cell cycle regulatory genes by modulating acetylation of Lys-10 (H3K9ac). Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.; Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.; Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression.; Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.; Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis.; Succinylation at Lys-80 (H3K79succ) by KAT2A takes place with a maximum frequency around the transcription start sites of genes. It gives a specific tag for epigenetic transcription activation. Desuccinylation at Lys-123 (H3K122succ) by SIRT7 in response to DNA damage promotes chromatin condensation and double-strand breaks (DSBs) repair.; Serine ADP-ribosylation constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Serine ADP-ribosylation at Ser-11 (H3S10ADPr) is mutually exclusive with phosphorylation at Ser-11 (H3S10ph) and impairs acetylation at Lys-10 (H3K9ac).

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Core component of nucleosome. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 is one of the five main histones involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

CITATIONS

  • Monounsaturated fatty acid-enriched olive oil exacerbates chronic alcohol-induced hepatic steatosis and liver injury in C57BL/6J mice

  • Songsong Zhu

    Identification of Human Temporomandibular Joint Fibrocartilage Stem Cells with Distinct Chondrogenic Capacity

  • Spermidine protects intestinal mucosal barrier function in mice colitis via the AhR/Nrf2 and AhR/STAT3 signaling pathways

  • Zhang Haizheng,et al

    Low-level Nd:YAG laser inhibiting inflammation and oxidative stress in human gingival fibroblasts via AMPK/SIRT3 axis

  • Zheng, F., Tao, Y., Liu, J., Geng, Z., Wang, Y., Wang, Y., Fu, S., Wang, W., Xie, C., Zhang, Y., & Gong, F.

    KCa3.1 Inhibition of Macrophages Suppresses Inflammatory Response Leading to Endothelial Damage in a Cell Model of Kawasaki Disease. Journal of inflammation research, 14, 719–735.

  • Guo, R., Zhu, J., Chen, L., Li, J., Ding, Q., Han, Q., Zheng, W., & Li, S.

    Dietary camellia seed oil attenuates liver injury in mice chronically exposed to alcohol

  • Wnt antagonist secreted frizzled‐related protein I (sFRP1) may be involved in the osteogenic differentiation of periodontal ligament cells in chronic apical periodontitis

  • Shen, X., Li, M., Wang, C., Liu, Z., Wu, K., Wang, A., Bi, C., Lu, S., Long, H., & Zhu, G.

    Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway

  • FHL3 promotes pancreatic cancer invasion and metastasis through preventing the ubiquitination degradation of EMT associated transcription factors

  • KMT2C deficiency promotes small cell lung cancer metastasis through DNMT3A-mediated epigenetic reprogramming

  • Tao, M., Zhu, W., Han, H., Liu, S., Liu, A., Li, S., Fu, H., & Tian, J.

    Mitochondrial proteomic analysis reveals the regulation of energy metabolism and reactive oxygen species production in Clematis terniflora DC. leaves under high-level UV-B radiation followed by dark treatmenttreatment

  • Liu Lin,et al

    NKG2D knockdown improves hypoxic-ischemic brain damage by inhibiting neuroinflammation in neonatal mice

  • Dong, J., Zhong, T., Xu, Z., Chen, H., Wang, X., Yang, L., Lou, Z., Xu, Y., Hou, T., Xu, R., Zhu, W., & Shao, J.

    Identification of Monobenzone as a Novel Potential Anti-Acute Myeloid Leukaemia Agent That Inhibits RNR and Suppresses Tumour Growth in Mouse Xenograft Model

  • Yafei Wu

    Fusobacterium nucleatum stimulates monocyte adhesion to and transmigration through endothelial cells

  • Xu, Y., Fang, H., Chen, Y., Tang, Y., Sun, H., Kong, Z., Yang, F., Kirschner-Schwabe, R., Zhu, L., Toker, A., Xiao, N., Zhou, B. S., & Li, H.

    The KRAS-G12D mutation induces metabolic vulnerability in B-cell acute lymphoblastic leukemia

  • Ling, Y. H., Wang, H., Han, M. Q., Wang, D., Hu, Y. X., Zhou, K., & Li, Y.

    Nucleoporin 85 interacts with influenza A virus PB1 and PB2 to promote its replication by facilitating nuclear import of ribonucleoprotein

  • Wang, H., Li, T., Wang, X., Guan, Y., Jiang, Y., Chen, S., Zou, S., & Duan, P.

    Mechanisms of sphingosine-1-phosphate (S1P) signaling on excessive stress-induced root resorption during orthodontic molar intrusion

  • Yizhen Wang

    Cathelicidin-WA attenuates LPS-induced inflammation and redox imbalance through activation of AMPK signaling

  • Qin, T. T., Li, Z. H., Li, L. X., Du, K., Yang, J. G., Zhang, Z. Q., Wu, X. X., & Ma, J. L.

    Sanguinarine, identified as a natural alkaloid LSD1 inhibitor, suppresses lung cancer cell growth and migration

  • Chong Chen;Xiao Shuai ; Yu Liu

    Epigenetic drug library screening identified an LSD1 inhibitor to target UTX-deficient cells for differentiation therapy

  • Supplementation with L-carnitine rescues sperm epigenetic changes in asthenospermic male semen with altered acetyl-L-carnitine levels

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