HRP Conjugated Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] - Loading control
Catalog# HA724276H
HRP Conjugated Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] - Loading control
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WB
-
Human
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Mouse
-
Rat
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Green monkey
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HRP
Overview
Product Name
HRP Conjugated Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] - Loading control
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Histone H3 aa 85-136/136.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB
Molecular Weight
Predicted band size: 15 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate.
Conjugation
HRP
Clone Number
JJ090-07
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000-1:20,000
Target
Function
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.
Background References
1. Wani S et al. Human SCP4 is a chromatin-associated CTD phosphatase and exhibits the dynamic translocation during erythroid differentiation. J Biochem 160:111-20 (2016).
2. Ni JZ et al. A transgenerational role of the germline nuclear RNAi pathway in repressing heat stress-induced transcriptional activation in C. elegans. Epigenetics Chromatin 9:3 (2016).
Subcellular Location
Nucleus, Chromosome.
UNIPROT
Synonyms
H3 histone family, member A antibody
H3/A antibody
H31_HUMAN antibody
H3FA antibody
Hist1h3a antibody
HIST1H3B antibody
HIST1H3C antibody
HIST1H3D antibody
HIST1H3E antibody
HIST1H3F antibody
ExpandImages
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Western blot analysis of Histone H3 on different lysates with Mouse anti-Histone H3 antibody (HA724276H) at 1/10,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: C6 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724276H, HRP) at 1/10,000 dilution was used in primary antibody dilution buffer (K1803) at room temperature for 1 hour.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Conjugate: unconjugated
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