Histone H3 Recombinant Antibody [6-A7-R] - Chicken IgY (Chimeric)

Usd: 385 Special Discount

Specification

100μl

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Catalog# HA601684

Histone H3 Recombinant Antibody [6-A7-R] - Chicken IgY (Chimeric)

Application

WB
IHC-P
IF-Tissue

Reactivity

Human
Mouse
Rat

BSA and Azide free

HA610463

Conjugation

unconjugated

Overview

Product Name

Histone H3 Recombinant Antibody [6-A7-R] - Chicken IgY (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human Histone H3 aa 1-50 / 136.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Target Molecular Weight

Predicted band size: 15 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, C6 (Rat glioma cell) cell lysate.

Conjugation

unconjugated

Clone Number

6-A7-R

Reactivity Data

Tested

Verified (internally validated)

Published

Reported in literature (not internally validated)

Predicted

Predicted reactive (based on sequence homology)

Not recommended

Not recommended (failed internal validation)

	WB	IHC-P	IF-Tissue
human
mouse
rat

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Lot Concentration Lookup

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

WB
1:5,000

IHC-P
1:200

IF-Tissue
1:500

Target

Function

The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Background References

1. Flanagan J.F., Mi L.-Z., Chruszcz M., Cymborowski M., Clines K.L., Kim Y., Minor W., Rastinejad F., Khorasanizadeh S."Double chromodomains cooperate to recognize the methylated histone H3 tail."Nature 438:1181-1185(2005)

2. "Arginine methylation of the histone H3 tail impedes effector binding."Iberg A.N., Espejo A., Cheng D., Kim D., Michaud-Levesque J., Richard S., Bedford M.T.J. Biol. Chem. 283:3006-3010(2008)

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P68431 human

SwissProt: P84243 human

SwissProt: Q16695 human

SwissProt: Q6NXT2 human

SwissProt: Q71DI3 human

SwissProt: P68433 mouse

SwissProt: P84228 mouse

SwissProt: Q6LED0 rat

Synonyms

H3 histone family member E pseudogene antibody

H3 histone family, member A antibody

H3/A antibody

H31_HUMAN antibody

H3F3 antibody

H3FA antibody

Hist1h3a antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

Expand

Images

Western blot analysis of Histone H3 on different lysates with Chicken anti-Histone H3 antibody (HA601684) at 1/5,000 dilution.

Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lane 5: C6 (Rat glioma cell) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601684, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Chicken IgY IgG-HRP (HA1026), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 15 kDa
Observed band size: 15 kDa
Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601684, 1/200, 1 hour at room temperature.
Secondary antibody: HA1146, 20 minutes at room temperature.
Application: Immunofluorescence (IF-tissue)

Species: Mouse
Tissue: Kidney
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601684, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Histone H3 Recombinant Antibody [6-A7-R] - Chicken IgY (Chimeric)

Usd: 385 Special Discount

Specification

Overview

Product Name

Antibody Type

Immunogen

Species Reactivity

Validated Applications

Target Molecular Weight

Positive Control

Conjugation

Clone Number

Reactivity Data

Product Features

Form

Concentration

Storage Instructions

Storage Buffer

Purification Method

Application Dilution

Target

Function

Background References

Subcellular Location

UNIPROT

Synonyms

Images

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