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HRP Conjugated Rabbit anti-Chicken IgY polyclonal Antibody

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Catalog# HA1026

HRP Conjugated Rabbit anti-Chicken IgY polyclonal Antibody

Application

  • ELISA

  • WB

Reactivity

  • Chicken

Conjugation

  • HRP

Overview

Product Name

HRP Conjugated Rabbit anti-Chicken IgY polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein.

Species Reactivity

Chicken

Validated Applications

ELISA, WB

Conjugation

HRP

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • ELISA

  • 1:5,000-1:10,000

  • WB

  • 1:500-1:2,000

Target

Function

Immunoglobulin Y (abbreviated as IgY) is a type of immunoglobulin which is the major antibody in bird, reptile, and lungfish blood. It is also found in high concentrations in chicken egg yolk. As with the other immunoglobulins, IgY is a class of proteins which are formed by the immune system in reaction to certain foreign substances, and specifically recognize them. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Background References

暂无

Synonyms

Immunoglobulin of yolk

Images

  • Western blot analysis of Rabbit anti Chicken IgY-HRP on Chicken IgY lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Rabbit anti-Chicken IgY-HRP Secondary Antibody (<a href="/products/HA1026" style="font-weight: bold;text-decoration: underline;">HA1026</a>) at 1:500 (Lane 1),1:1,000 (Lane 2)dilution was used for 2 hour at room temperature.

    Western blot analysis of Rabbit anti Chicken IgY-HRP on Chicken IgY lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Rabbit anti-Chicken IgY-HRP Secondary Antibody (HA1026) at 1:500 (Lane 1),1:1,000 (Lane 2)dilution was used for 2 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"