Formyl-Histone H3 (K122) Recombinant Rabbit Monoclonal Antibody [PSH27-72]

Usd: 385 Special Discount

Specification

100μl

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Catalog# HA724717

Formyl-Histone H3 (K122) Recombinant Rabbit Monoclonal Antibody [PSH27-72]

Application

WB
IHC-P

Reactivity

Human
Mouse
Rat

Conjugation

unconjugated

Overview

Product Name

Formyl-Histone H3 (K122) Recombinant Rabbit Monoclonal Antibody [PSH27-72]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 15 kDa

Positive Control

A549 (Human lung adenocarcinoma cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate.

Conjugation

unconjugated

Clone Number

PSH27-72

Reactivity Data

Tested

Verified (internally validated)

Published

Reported in literature (not internally validated)

Predicted

Predicted reactive (based on sequence homology)

Not recommended

Not recommended (failed internal validation)

	WB	IHC-P
human
mouse
rat

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Lot Concentration Lookup

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

WB
1:500

IHC-P
1:500

Target

Function

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to methylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.

Background References

1. Wilson J.P. et. al. Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants. Mol. Cell. Proteomics 10:M110.001198-M110.001198(2011).

Subcellular Location

Chromosome, Nucleosome core, Nucleus.

UNIPROT

SwissProt: P68431 Human

SwissProt: P84243 Human

SwissProt: Q16695 Human

SwissProt: Q71DI3 Human

SwissProt: Q6NXT2 Human

Synonyms

H3 histone family member E pseudogene antibody

H3 histone family, member A antibody

H3/A antibody

H31_HUMAN antibody

H3F3 antibody

H3FA antibody

Hist1h3a antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

Expand

Images

Western blot analysis of Formyl-Histone H3 (K122) on different lysates with Rabbit anti-Formyl-Histone H3 (K122) antibody (HA724717) at 1/500 dilution.

Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes ; ECL: K1802

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724717, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 15 kDa
Observed band size: 15 kDa
Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Colon
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724717, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Colon
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724717, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Colon
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724717, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Formyl-Histone H3 (K122) Recombinant Rabbit Monoclonal Antibody [PSH27-72]

Usd: 385 Special Discount

Specification

Overview

Product Name

Antibody Type

Species Reactivity

Validated Applications

Target Molecular Weight

Positive Control

Conjugation

Clone Number

Reactivity Data

Product Features

Form

Concentration

Storage Instructions

Storage Buffer

Isotype

Purification Method

Application Dilution

Target

Function

Background References

Subcellular Location

UNIPROT

Synonyms

Images

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