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HRP Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody

Usd: 30

Specification

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Catalog# HA1146

HRP Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody

Application

  • WB

  • ELISA

Reactivity

  • Chicken

Conjugation

  • HRP

Overview

Product Name

HRP Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody

Antibody Type

Goat Polyclonal Antibody

Immunogen

Recombinant protein.

Species Reactivity

Chicken

Validated Applications

WB, ELISA

Conjugation

HRP

Reactivity Data

WB ELISA
Chicken
Species Independent
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:10,000-1:50,000

Target

Function

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Background References

暂无

Images

  • Western blot analysis of SOX2 on different lysates with Chicken anti-SOX2 antibody (<a href="/products/HA601499" style="font-weight: bold;text-decoration: underline;">HA601499</a>) at 1/5,000 dilution.<br /><br />Lane 1: NCCIT cell lysate<br />Lane 2: F9 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 34 kDa<br />Observed band size: 36 kDa<br /><br />Exposure time: 40 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601499" style="font-weight: bold;text-decoration: underline;">HA601499</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat anti-Chicken IgY - HRP Secondary Antibody (<a href="/products/HA1146" style="font-weight: bold;text-decoration: underline;">HA1146</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SOX2 on different lysates with Chicken anti-SOX2 antibody (HA601499) at 1/5,000 dilution.

    Lane 1: NCCIT cell lysate
    Lane 2: F9 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 34 kDa
    Observed band size: 36 kDa

    Exposure time: 40 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601499) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat anti-Chicken IgY - HRP Secondary Antibody (HA1146) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"