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Cathepsin D Recombinant Rabbit Monoclonal Antibody [SU0360]

Usd: 205

Specification

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Catalog# ET1608-49

Cathepsin D Recombinant Rabbit Monoclonal Antibody [SU0360]

Application

  • WB

  • IF-Cell

  • IHC-P

  • IP

  • mIHC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cathepsin D Recombinant Rabbit Monoclonal Antibody [SU0360]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Cathepsin D aa 363-412 / 412.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, IP, mIHC

Target Molecular Weight

Predicted band size: 45/28 kDa

Positive Control

HepG2 cell lysate, MCF7 cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, C6 cell lysate, Rat brain tissue lysate, human breast cancer tissue, human pancreas tissue, PANC-1, AGS.

Conjugation

unconjugated

Clone Number

SU0360

RRID

AB_3069810

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P IF-Cell IF-Tissue IP
human
Tested Tested
Tested Tested
Tested Tested
 
Tested Tested
mouse
Tested Tested
Not Recommended
 
Tested Tested
 
rat
Tested Tested
Not Recommended
Not Recommended
 
 

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:100-1:500

  • IHC-P

  • 1:5,000-1:10,000

  • IP

  • Use at an assay dependent concentration.

  • mIHC

  • 1:1,000

Target

Function

Cathepsin D is a protein that in humans is encoded by the CTSD gene. This gene encodes a lysosomal aspartyl protease composed of a protein dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. Cathepsin D is an aspartic endo-protease that is ubiquitously distributed in lysosomes. The main function of cathepsin D is to degrade proteins and activate precursors of bioactive proteins in pre-lysosomal compartments. This proteinase, which is a member of the peptidase A1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of the CTSD gene is initiated from several sites, including one that is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. Homozygous deletion of the CTSD gene leads to early lethality in the postnatal phase. Deficiency of CTSD gene has been reported an underlying cause of neuronal ceroid lipofuscinosis (NCL).

Background References

1. Santaguida S et al. Aneuploidy-induced cellular stresses limit autophagic degradation. Genes Dev 29:2010-21 (2015).

2. Shen C et al. Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network. Proteomics 12:1917-27 (2012).

Sequence Similarity

Belongs to the peptidase A1 family.

Tissue Specificity

Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level).

Post-translational Modification

N- and O-glycosylated.; Undergoes proteolytic cleavage and activation by ADAM30.; As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified.

Subcellular Location

Extracellular space, Lysosome, Melanosome.

UNIPROT

SwissProt: P07339 Human

SwissProt: P18242 Mouse

SwissProt: P24268 Rat

Synonyms

CatD antibody

CATD_HUMAN antibody

Cathepsin D antibody

Cathepsin D heavy chain antibody

CD antibody

Ceroid lipofuscinosis neuronal 10 antibody

CLN10 antibody

CPSD antibody

ctsd antibody

Epididymis secretory sperm binding protein Li 130P antibody

Expand

Images

  • Western blot analysis of Cathepsin D on different lysates with Rabbit anti-Cathepsin D antibody (<a href="/products/ET1608-49" style="font-weight: bold;text-decoration: underline;">ET1608-49</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (15 µg/Lane)<br />Lane 2: MCF7 cell lysate (15 µg/Lane)<br />Lane 3: Neuro-2a cell lysate (15 µg/Lane)<br />Lane 4: Mouse brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 45/28 kDa<br />Observed band size: 45/28 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-49" style="font-weight: bold;text-decoration: underline;">ET1608-49</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cathepsin D on different lysates with Rabbit anti-Cathepsin D antibody (ET1608-49) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate (15 µg/Lane)
    Lane 2: MCF7 cell lysate (15 µg/Lane)
    Lane 3: Neuro-2a cell lysate (15 µg/Lane)
    Lane 4: Mouse brain tissue lysate (20 µg/Lane)

    Predicted band size: 45/28 kDa
    Observed band size: 45/28 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-49) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Cathepsin D on different lysates with Rabbit anti-Cathepsin D antibody (<a href="/products/ET1608-49" style="font-weight: bold;text-decoration: underline;">ET1608-49</a>) at 1/1,000 dilution.<br /><br />Lane 1: C6 cell lysate (20 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 3: Rat brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 45/28 kDa<br />Observed band size: 45/28 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-49" style="font-weight: bold;text-decoration: underline;">ET1608-49</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cathepsin D on different lysates with Rabbit anti-Cathepsin D antibody (ET1608-49) at 1/1,000 dilution.

    Lane 1: C6 cell lysate (20 µg/Lane)
    Lane 2: Mouse brain tissue lysate (20 µg/Lane)
    Lane 3: Rat brain tissue lysate (20 µg/Lane)

    Predicted band size: 45/28 kDa
    Observed band size: 45/28 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-49) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Cathepsin D antibody (<a href="/products/ET1608-49" style="font-weight: bold;text-decoration: underline;">ET1608-49</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1608-49" style="font-weight: bold;text-decoration: underline;">ET1608-49</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Cathepsin D antibody (ET1608-49) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-49) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Cathepsin D antibody (<a href="/products/ET1608-49" style="font-weight: bold;text-decoration: underline;">ET1608-49</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1608-49" style="font-weight: bold;text-decoration: underline;">ET1608-49</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Cathepsin D antibody (ET1608-49) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-49) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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