ERK1/2 Recombinant Rabbit Monoclonal Antibody [SA43-03] (ET1601-29)
Catalog# ET1601-29
Rabbit Monoclonal to ERK1/2
-
WB
-
IF-Cell
-
IF-Tissue
-
IP
-
FC
-
IHC-P
-
IHC-Fr
-
Human
-
Mouse
-
Rat
-
Zebrafish
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HepG2 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 43/41 kDa
Observed band size: 43/41 kDa
Exposure time: 19 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-29) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ERK1/2 Recombinant Rabbit Monoclonal Antibody [SA43-03] (ET1601-29)
Immunogen
Recombinant protein within human erk2 aa 180-379.
Host
Rabbit
Positive Control
HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, Jurkat, human breast carcinoma tissue, mouse esophagus tissue, mouse stomach tissue, Hela, MCF-7, NIH/3T3, A549.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SA43-03
RRID
APPLICATION DILUTION
-
WB
-
1:5,000
-
IF-Cell
-
1:2,000
-
IHC-P
-
1:20,000
-
IHC-Fr
-
1:50
-
IF-Tissue
-
1:50
-
FC
-
1:1,000
-
IP
-
Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 43/41 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ERK1/2
SYNONYMS
ERK 1 antibody;ERK 2 antibody;ERK-2 antibody;ERK1 antibody;erk1/2 antibody;ERK2 antibody;ERT1 antibody;ERT2 antibody;Extracellular signal regulated kinase 1 antibody;Extracellular signal-regulated kinase 2 antibody;MAP kinase 1 antibody;MAP kinase 2 antibody;MAP kinase isoform p42 antibody;MAP kinase isoform p44 antibody;MAPK 1 antibody;MAPK 2 antibody;MAPK 3 antibody;Mapk1 antibody;MAPK2 antibody;MAPK3 antibody;Mitogen-activated protein kinase 1 antibody;Mitogen-activated protein kinase 2 antibody;MK01_HUMAN antibody;p38 antibody;p40 antibody;p41 antibody;p42-MAPK antibody;PRKM 2 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
The first mitogen-activated protein kinase to be discovered was ERK1 (MAPK3) in mammals. Since ERK1 and its close relative ERK2 (MAPK1) are both involved in growth factor signaling, the family was termed "mitogen-activated". With the discovery of other members, even from distant organisms (e.g. plants), it has become increasingly clear that the name is a misnomer, since most MAPKs are actually involved in the response to potentially harmful, abiotic stress stimuli (hyperosmosis, oxidative stress, DNA damage, low osmolarity, infection, etc.). Because plants cannot "flee" from stress, terrestrial plants have the highest number of MAPK genes per organism ever found[citation needed]. Thus the role of mammalian ERK1/2 kinases as regulators of cell proliferation is not a generic, but a highly specialized function.
CITATIONS
-
Alkannin induces cytotoxic autophagy and apoptosis by promoting ROS-mediated mitochondrial dysfunction and activation of JNK pathway
-
WB
-
Citation
-
-
Li, Y., Li, D., You, L., Deng, T., Pang, Q., Meng, X., & Zhu, B.
dCas9-Based PDGFR-β Activation ADSCs Accelerate Wound Healing in Diabetic Mice through Angiogenesis and ECM Remodeling
-
WB
-
Mouse
-
Human
-
Citation
-
-
Zenghua Sheng, Xu Cao, Ya-nan Deng, Xinyu Zhao, Shufang Liang
SUMOylation of AnxA6 facilitates EGFR-PKCα complex formation to suppress epithelial cancer growth
-
WB
-
Human
-
Citation
-
-
Wang Jinqing,et al
IGFBP7 promotes gastric cancer by facilitating epithelial-mesenchymal transition of gastric cells
-
WB
-
Human
-
Citation
-
-
Wang Ke,et al
CD147‐K148me2‐Driven Tumor Cell‐Macrophage Crosstalk Provokes NSCLC Immunosuppression via the CCL5/CCR5 Axis
-
WB
-
Human
-
Citation
-
-
Liao Siqi,et al
Mito-LND and (E)-Akt inhibitor-IV: novel compounds inducing endoplasmic reticulum stress and ROS accumulation against hepatocellular carcinoma
-
WB
-
Human
-
Citation
-
-
He, Y., Wang, X., Lu, W., Zhang, D., Huang, L., Luo, Y., Xiong, L., Li, H., Zhang, P., Li, Q., & Liang, S.
PGK1 contributes to tumorigenesis and sorafenib resistance of renal clear cell carcinoma via activating CXCR4/ERK signaling pathway and accelerating glycolysis
-
WB
-
Human
-
Citation
-
-
Li Shenghao,et al
PD-1/PD-L1 Interaction Upregulates YAP1 Expression in HepG2 Cells Through MAPK/ERK Pathway
-
WB
-
Human
-
Citation
-
-
Lycium barbarum polysaccharide‐glycoprotein promotes osteogenesis in hPDLSCs via ERK activation
-
WB
-
Human
-
Citation
-
-
Identification of a Specific Phage as Growth Factor Alternative Promoting the Recruitment and Differentiation of MSCs in Bone Tissue Regeneration
-
WB
-
Mouse
-
Citation
-
-
Orexin-A exerts neuroprotective effect in experimental intracerebral hemorrhage by suppressing autophagy via OXR1-mediated ERK/mTOR signaling pathway
-
WB
-
rat
-
Citation
-
-
Yin, Y., Wang, J., Xu, X., Zhou, B., Chen, S., Qin, T., & Peng, D.
Riboflavin as a Mucosal Adjuvant for Nasal Influenza Vaccine
-
WB
-
Mouse
-
Citation
-
-
Zhang, J. J., Song, C. G., Dai, J. M., Zhang, X. Q., Lin, P., Li, L., Yang, X. M., & Chen, Z. N.
Inhibition of mu-opioid receptor suppresses proliferation of hepatocellular carcinoma cells via CD147-p53-MAPK cascade signaling pathway. American journal of translational research, 13(5), 3967–3986.
-
WB
-
Human
-
Citation
-
-
Bao Mengyuan,et al
Coptidis rhizoma extract alleviates oropharyngeal candidiasis by gC1qR-EGFR/ERK/c-Fos axis-induced endocytosis of oral epithelial cells
-
WB
-
Mouse
-
Citation
-
-
PER3 plays anticancer roles in the oncogenesis and progression of breast cancer via regulating MEK/ERK signaling pathway
-
WB
-
Human
-
Citation
-
-
NSD3, a member of nuclear receptor-binding SET domain family, is a potential prognostic biomarker for pancreatic cancer
-
WB
-
Human
-
Citation
-
-
Chen Jianghong,et al
Enhancing the therapeutic efficacy of gefitinib on subcutaneously transplanted SKOV3 ovarian cancer tumors in nude mice via ultrasound‑stimulated microbubble cavitation
-
WB
-
Mouse
-
Citation
-
-
Ji Zhongyin,et al
Injectable hydrogel encapsulating siMMP13 with anti-ROS and anti-apoptotic functions for osteoarthritis treatment
-
WB
-
Mouse
-
Citation
-
-
MSCs-derived exosomes attenuate acute brain injury and inhibit microglial inflammation by reversing CysLT2R-ERK1/2 mediated microglia M1 polarization
-
WB
-
Rat
-
Citation
-
-
Yang, Y., Zhou, X., Li, Y., Chen, A., Liang, W., Liang, G., Huang, B., Li, Q., & Jin, D.
CXCL2 attenuates osteoblast differentiation by inhibiting the ERK1/2 signaling pathway
-
WB
-
Mouse
-
Citation
-
-
Zhang, H., Zhang, W. W., Mo, C. Y., Dong, M. D., Jia, K. T., Liu, W., & Yi, M. S.
Production of functional sperm from in vitro-cultured premeiotic spermatogonia in a marine fish
-
WB
-
Citation
-
-
Tang D, Yan Y, Li Y, et al
Targeting DAD1 gene with CRISPR-Cas9 system transmucosally delivered by fluorinated polylysine nanoparticles for bladder cancer intravesical gene therapy
-
WB
-
Human
-
Citation
-
-
Chen Jiahe,et al
ETS1 and RBPJ transcriptionally regulate METTL14 to suppress TGF-β1-induced epithelial-mesenchymal transition in human bronchial epithelial cells
-
WB
-
Human
-
Mouse
-
Citation
-
-
Chunling Ma;Bin Cong
Molecular hydrogen attenuates methamphetamine-induced behavioral sensitization and activation of ERK-ΔFosB signaling in the mouse nucleus accumbens
-
WB
-
Mouse
-
Citation
-
-
Chen, R., Huang, M., Yang, X., Chen, X. H., Shi, M. Y., Li, Z. F., Chen, Z. N., & Wang, K.
CALR-TLR4 Complex Inhibits Non-Small Cell Lung Cancer Progression by Regulating the Migration and Maturation of Dendritic Cells
-
WB
-
Mouse
-
Citation
-
-
Li Zong,et al
Schisandrin A alleviates non-alcoholic fatty liver disease by improving liver metabolic disorders and suppressing the GSK3β signaling pathway
-
WB
-
Mouse
-
Citation
-
-
Periplaneta americana extract promotes osteoblast differentiation of human alveolar bone marrow mesenchymal stem cells
-
WB
-
Human
-
Citation
-
-
Mettl3 Deficiency Sustains Long-Chain Fatty Acid Absorption through Suppressing Traf6-Dependent Inflammation Response
-
WB
-
Pig
-
Citation
-
-
Xiao, X., Zou, S., & Chen, J.
Cyclic tensile force modifies calvarial osteoblast function via the interplay between ERK1/2 and STAT3
-
WB
-
ICC
-
Mouse
-
Citation
-
-
Chao Shen
Exopolysaccharide from Cryptococcus heimaeyensis S20 induces autophagic cell death in non-small cell lung cancer cells via ROS/p38 and ROS/ERK signalling
-
WB
-
IHC
-
human
-
Citation
-
-
Li Jing,et al
Aquaporin-3 is down-regulated by LMP1 in nasopharyngeal carcinoma cells to regulate cell migration and affect EBV latent infection
-
WB
-
Human
-
Citation
-
-
Antibacterial and Angiogenic (2A) Bio-Heterojunctions Facilitate Infectious Ischemic Wound Regeneration via an Endogenous-Exogenous Bistimulatory Strategy
-
WB
-
Mouse
-
Citation
-
-
Xiawei Wei
Napabucasin,a novel inhibitor of STAT3,inhibits growth and synergises with doxorubicin in diffuse large B-cell lymphoma
-
WB
-
human
-
Citation
-
-
Xie Binqing,et al
Cyclic tensile stress promotes osteogenic differentiation via upregulation of Piezo1 in human dental follicle stem cells
-
WB
-
Human
-
Citation
-
-
Bai Mengru,et al
Production of recombinant human epidermal growth factor fused with HaloTag protein and characterisation of its biological functions
-
WB
-
Human
-
Citation
-
-
Hyperglycemia modulates M1/M2 macrophage polarization via reactive oxygen species overproduction in ligature-induced periodontitis
-
WB
-
Mouse
-
Citation
-
-
Zhu, M., Miao, S., Zhou, W., Elnesr, S. S., Dong, X., & Zou, X.
MAPK, AKT/FoxO3a and mTOR pathways are involved in cadmium regulating the cell cycle, proliferation and apoptosis of chicken follicular granulosa cells. Ecotoxicology and environmental safety, 214, 112091.
-
WB
-
Hen
-
Citation
-
-
D-mannose induces TFE3-dependent lysosomal degradation of EGFR and inhibits the progression of NSCLC
-
WB
-
Human
-
Citation
-
-
Bai Hongxia,et al
MiRNA-145-5p inhibits gastric cancer progression via the serpin family E member 1-extracellular signal-regulated kinase-1/2 axis
-
WB
-
Human
-
Citation
-
-
Taoxia Wang,et al
Hypomethylation of theLncRNA H19 promoteraccelerates osteogenicdifferentiation of vascularsmooth muscle cells byactivating the Erk1/2pathways
-
WB
-
Human
-
Citation
-
-
Huang Z , Dai L, Peng F,et al
mTOR signaling pathway regulates embryonic development and rapid growth of triploid crucian carp
-
WB
-
Mouse
-
Citation
-
-
Mengdi Yang ,et al
IDO1 inhibitors are synergistic with CXCL10 agonists in inhibiting colon cancer growth
-
WB
-
Human
-
Citation
-
-
Intracellular angiopoietin-1 promotes TKI-resistance via activation of JAK/STAT5 pathway in chronic myeloid leukemia
-
WB
-
Human
-
Citation
-
-
SETDB1-mediated CD147-K71 di-methylation promotes cell apoptosis in non-small cell lung cancer
-
WB
-
Human
-
Citation
-
-
MDM2 upregulation induces mitophagy deficiency via Mic60 ubiquitination in fetal microglial inflammation and consequently neuronal DNA damage caused by exposure to ZnO-NPs during pregnancy
-
WB
-
Mouse
-
Citation
-
-
LncRNA RP11-93B14. 5 promotes gastric cancer cell growth through PI3K/AKT signaling pathway
-
WB
-
Human
-
Citation
-
-
Wang, C., Wei, S., Liu, B., Wang, F., Lu, Z., Jin, M., & Wang, Y.
Maternal consumption of a fermented diet protects offspring against intestinal inflammation by regulating the gut microbiota
-
WB
-
Pig
-
Citation
-
-
Purification of recombinant human fibroblast growth factor 13 in E. coli and its molecular mechanism of mitogenesis
-
WB
-
Mouse
-
Citation
-
-
Pang, Y., Zhao, L., Ji, X., Guo, K., & Yin, X.
Analyses of Transcriptomics upon IL-1β-Stimulated Mouse Chondrocytes and the Protective Effect of Catalpol through the NOD2/NF-κB/MAPK Signaling Pathway
-
WB
-
Mouse
-
Citation
-
-
J Qian,et al
The CD163/TWEAK/Fn14 axis: A potential therapeutic target for alleviating inflammatory bone loss
-
WB
-
Mouse
-
Citation
-
-
Di Guohui,et al
Extracellular Vesicles Containing GAS6 Protect the Liver from Ischemia-Reperfusion Injury by Enhancing Macrophage Efferocytosis via MerTK-ERK-COX2 Signaling
-
WB
-
Citation
-
-
Downregulation of a CT10 regulator of kinase (Crk) promotes the formation of diapause embryos in the brine shrimp Artemia
-
WB
-
Brine shrimp Artemia
-
Citation
-
-
miR-27a-3p relieves heat stress-induced mitochondrial damage and aberrant milk protein synthesis through MEK/ERK pathway in BMECs
-
WB
-
Mouse
-
Citation
-
-
Fang T, Jiang J, Yu W, Li R, Tian H
DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c-Myc signaling pathway
-
WB
-
Mouse
-
Citation
-
-
Transient Receptor Potential Melastatin 7 Promotes Vascular Adventitial Fibroblasts Phenotypic Transformation and Inflammatory Reaction Induced by Mechanical Stretching Stress via p38 MAPK/JNK Pathway
-
WB
-
Rat
-
Citation
-
-
Pan, G., Zhong, M., Yao, J., Tan, J., Zheng, H., Jiang, Y., Tang, Y., Zhou, H., Qin, D., Yu, X., Liu, L., Li, Z., Lin, Z., Jiang, Y., Xu, B., & Zha, J.
Orelabrutinib and venetoclax synergistically induce cell death in double hit lymphoma by interfering with the crosstalk between the PI3K/AKT and p38/MAPK signaling
-
WB
-
Citation
-
-
Yuan, Y., Wang, X., Li, J., Han, L., Du, H., Sun, Y., Yang, P., Zhou, Z., Gu, M., Lu, Y., & Shen, C.
Single-Cell Sequencing Yields Insights in the Evolution of Foot-and-Mouth Disease Virus Persistent Infection
-
WB
-
Hamster
-
Citation
-
-
Lu Py,et al
Effect and mechanism of recombinant human fibroblast growth factor 18 on osteoporosis in OVX mice
-
WB
-
Mouse
-
Citation
-
-
Intermittent parathyroid hormone promotes cementogenesis in a PKA- and ERK1/2-dependent manner
-
WB
-
Mouse
-
Citation
-
-
Chen Liangmin,et al
Psoralen and Isopsoralen, Two Estrogen-Like Natural Products from Psoraleae Fructus, Induced Cholestasis via Activation of ERK1/2
-
WB
-
Zebrafish
-
Citation
-
-
Tao Guoa,Fengjie Huang
Sini decoction ameliorates sepsis-induced acute lung injury via regulating ACE2-Ang (1-7)-Mas axis and inhibiting the MAPK signaling pathway
-
WB
-
human
-
Citation
-
-
Ji, X., Du, W., Che, W., Wang, L., & Zhao, L.
Apigenin Inhibits the Progression of Osteoarthritis by Mediating Macrophage Polarization
-
WB
-
Mouse
-
Citation
-
-
Scutellarin ameliorates diabetic nephropathy via TGF-β1 signaling pathway
-
WB
-
Mouse
-
Citation
-
-
CD147 Mediates 5-Fluorouracil Resistance in Colorectal Cancer by Reprogramming Glycolipid Metabolism
-
WB
-
Human
-
Citation
-
-
Fan, R., Chen, C., Hu, J., Mu, M., Chuan, D., Chen, Z., Guo, G., & Xu, J.
Multifunctional gold nanorods in low-temperature photothermal interactions for combined tumor starvation and RNA interference therapy
-
WB
-
Mouse
-
Citation
-
-
An oral phenylacrylic acid derivative suppressed hepatic stellate cell activation and ameliorated liver fibrosis by blocking TGF‐β1 signalling
-
WB
-
Human
-
Citation
-
-
PACAP inhibition alleviates neuropathic pain by modulating Nav1. 7 through the MAPK/ERK signaling pathway in a rat model of chronic constriction injury
-
WB
-
Rat
-
Citation
-
-
Li, S., Liu, Y., Zhang, T., Lin, S., Shi, S., He, J., Xie, Y., Cai, X., Tian, T., & Lin, Y.
A Tetrahedral Framework DNA-Based Bioswitchable miRNA Inhibitor Delivery System: Application to Skin Anti-Aging
-
WB
-
Mouse
-
Citation
-
Newsletter
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.