Paxillin Recombinant Rabbit Monoclonal Antibody [SY23-02] (ET1607-22)
Catalog# ET1607-22
Rabbit Monoclonal to Paxillin
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: PANC-1 cell lysate
Lane 4: RAW264.7 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-22) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Paxillin Recombinant Rabbit Monoclonal Antibody [SY23-02] (ET1607-22)
Immunogen
Synthetic peptide within human paxillin aa 1-59 / 591.
Host
Rabbit
Positive Control
HeLa cell lysate, A431 cell lysate, PANC-1 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Rat testis tissue lysate, SK-OV-3, C6, human kidney tissue, mouse testis tissue, human breast carcinoma tissue, mouse ovary tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY23-02
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 65 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Paxillin
SYNONYMS
FLJ16691 antibody;FLJ23042 antibody;Paired box protein Pax 1 antibody;PAX 1 antibody;PAX1 antibody;PAXI_HUMAN antibody;Paxillin alpha antibody;Paxillin antibody;PXN antibody;PXN protein antibody
SEQUENCE SIMILARITIES
Belongs to the paxillin family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility.
SUBCELLULAR LOCATION
Cytoplasm, Cell junction.
FUNCTION
Paxillin is a focal adhesion phosphoprotein that is localized to the cytoskeleton. Phosphorylation of paxillin has been shown to occur in response to PDGF treatment, v-Src transformation or cross-linking of integrins. FAK (focal adhesion kinase) and PYK2 have been shown to phosphorylate paxillin. FAK phosphorylates paxillin specifically on Tyr 118 in vitro. However, FAK phosphorylation does not seem to be required for the recruitment of paxillin to cell adhesion sites. Paxillin may play a role in signal transduction, regulation of cell morphology and the recruitment of structural and signaling molecules to focal adhesions. It has been shown that the amount of paxillin is reduced in mitotic cells by proteolytic downregulation and that paxillin is alternatively phosphorylated on serine rather than on tyrosine and serine during mitosis.
CITATIONS
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The virulence modulator PA-X protein has minor effect on the pathogenicity of the highly pathogenic H7N9 avian influenza virus in mice
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WB
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human
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Citation
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