DPPA2 Rabbit Polyclonal Antibody
Catalog# 0731-4
DPPA2 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Mouse
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unconjugated
Overview
Product Name
DPPA2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within mouse DPPA2 aa 1-50 / 301.
Species Reactivity
Mouse
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 34 kDa
Positive Control
F9, human colon tissue, human testis tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:2,000
Target
Function
DPPA2 (developmental pluripotency associated 2) is an embryo-cancer antigen that is coexpressed with cancer-testis antigens in non-small cell lung cancer. Dppa2 is closely linked SAP motif genes restricted to pluripotent cells and the germ line. It may play a role in maintaining cell pluripotentiality and in embryo preimplantation development.
Background References
1. Bortvin A., Eggan K., Skaletsky H., etc. "Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nuclei."; Development 130:1673-1680(2003).
2. Du J., Lin G., Nie Z.Y., Lu G.X.; "Molecular cloning and characterization analysis of HPESCRG1, a novel gene expressed specifically in human embryonic stem cell.";Zhonghua Yi Xue Yi Chuan Xue Za Zhi 21:542-547(2004).Kawai J."The transcriptional landscape of the mammalian genome.";Science 309:1559-1563(2005).
Tissue Specificity
Not detected in adult tissues.
Subcellular Location
Nucleus
UNIPROT
Synonyms
cancer/testis antigen 100 antibody
CT100 antibody
Developmental Pluripotency Associated 2 antibody
developmental pluripotency-associated gene 2 antibody
developmental pluripotency-associated protein 2 antibody
ECAT15-2 antibody
embryonic stem cell (ESC) associated transcript 15-2 antibody
PESCRG1 antibody
Pluripotent embryonic stem cell related protein 1 antibody
Pluripotent embryonic stem cell-related gene 1 antibody
ExpandImages
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Western blot analysis on F9 using anti-DPPA2 polyclonal antibody
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Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-DPPA2 antibody (0731-4) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0731-4) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DPPA2 antibody (0731-4) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0731-4) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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