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TIE-1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# 0804-11

TIE-1 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TIE-1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Human TIE-1 aa 23-72 / 1,138.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 125 kDa

Positive Control

HUVEC cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HepG2, SW620.

Conjugation

unconjugated

RRID

AB_3068651

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:50-1:200

Target

Function

Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 also known as TIE1 is an angiopoietin receptor which in humans is encoded by the TIE1 gene. TIE1 is a cell surface protein expressed exclusively in endothelial cells, however it has also been shown to be expressed in immature hematopoietic cells and platelets. TIE1 upregulates the cell adhesion molecules (CAMs) VCAM-1, E-selectin, and ICAM-1 through a p38-dependent mechanism. Attachment of monocyte derived immune cells to endothelial cells is also enhanced by TIE1 expression. TIE1 has a proinflammatory effect and may play a role in the endothelial inflammatory diseases such as atherosclerosis.

Background References

暂无

Sequence Similarity

Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.

Tissue Specificity

Specifically expressed in developing vascular endothelial cells.

Post-translational Modification

Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.

Subcellular Location

Cell membrane. Membrane.

UNIPROT

SwissProt: P35590 Human

SwissProt: Q06806 Mouse

Entrez Gene: 89806 Rat

Synonyms

JKT 14 antibody

JTK14 antibody

TIE antibody

Tie1 antibody

TIE1_HUMAN antibody

Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody

Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody

Tyrosine protein kinase receptor Tie 1 antibody

Tyrosine-protein kinase receptor Tie-1 antibody

Images

  • Western blot analysis of TIE-1 on different lysates with Rabbit anti-TIE-1 antibody (<a href="/products/0804-11" style="font-weight: bold;text-decoration: underline;">0804-11</a>) at 1/1,000 dilution.<br /><br />Lane 1: HUVEC cell lysate<br />Lane 2: K-562 cell lysate<br />Lane 3: NIH/3T3 cell lysate<br />Lane 4: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 125 kDa<br />Observed band size: 125 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/0804-11" style="font-weight: bold;text-decoration: underline;">0804-11</a>) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TIE-1 on different lysates with Rabbit anti-TIE-1 antibody (0804-11) at 1/1,000 dilution.

    Lane 1: HUVEC cell lysate
    Lane 2: K-562 cell lysate
    Lane 3: NIH/3T3 cell lysate
    Lane 4: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 125 kDa
    Observed band size: 125 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0804-11) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining Tie-1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Tie-1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Tie-1 in SW620 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Tie-1 in SW620 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation