Insulin B Chain Rabbit Polyclonal Antibody
Catalog# 0807-11
Insulin B Chain Rabbit Polyclonal Antibody
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IHC-P
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ELISA
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IF-Tissue
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Insulin B Chain Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Full length human Insulin B Chain protein.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-P, ELISA, IF-Tissue
Molecular Weight
Predicted band size: 12 kDa
Positive Control
Human pancreas tissue, mouse pancreas tissue, rat pancreas tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IHC-P
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1:2,000-1:10,000
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ELISA
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1:5,000
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IF-Tissue
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1:2,000
Target
Function
Insulin is a hormone with extensive effects on both metabolism and several other body systems. It causes most of the body's cells to take up glucose from the blood (including liver, muscle, and fat tissue cells), storing it as glycogen in the liver and muscle, and stops use of fat as an energy source. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide.
Background References
1. Bell G.I., Pictet R.L., Rutter W.J., Cordell B., Tischer E., Goodman H.M.;"Sequence of the human insulin gene.";Nature 284:26-32(1980).
2. Nicol D.S.H.W., Smith L.F.;"Amino-acid sequence of human insulin.";Nature 187:483-485(1960).
3. Chang X., Joergensen A.M., Bardrum P., Led J.J.;"Solution structures of the R6 human insulin hexamer."; Biochemistry 36:9409-9422(1997).
Sequence Similarity
Belongs to the insulin family.
Subcellular Location
Secreted.
Synonyms
IDDM antibody
IDDM1 antibody
IDDM2 antibody
ILPR antibody
ins antibody
INS_HUMAN antibody
Insulin A chain antibody
Insulin B chain antibody
IRDN antibody
MODY11 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded human pancreas tissue labeling Insulin B Chain with Rabbit anti-Insulin B Chain antibody (0807-11) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (0807-11, green) at 1/2,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling Insulin B Chain with Rabbit anti-Insulin B Chain antibody (0807-11) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (0807-11, green) at 1/2,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded rat pancreas tissue labeling Insulin B Chain with Rabbit anti-Insulin B Chain antibody (0807-11) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (0807-11, green) at 1/2,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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