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TMEM132B Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# 0903-1

TMEM132B Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

TMEM132B Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within N-terminal Human TMEM132B.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 119 kDa

Positive Control

HeLa cell lysate, SK-Br-3 cell lysate, U-87 MG cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

RRID

AB_3068698

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

TMEM132B is a member of TMEM132 family. Total of five members, namely TMEM132 A, B, C, D, E, were found. TMEM132A, also known as HSPA5-binding protein 1, may play a role in embryonic and postnatal development of the brain. TMEM132B as TMEM132A is also the single transmembrane protein, which has three isoforms: 120kDa, 66kDa and 55kDa.

Background References

1. Wang G et al. The TMEM132B-GABA(A) receptor complex controls alcohol actions in the brain. Cell. 2024 Nov

Sequence Similarity

Belongs to the TMEM132 family.

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q14DG7 Human

Entrez Gene: 208151 Mouse

Entrez Gene: 304458 Rat

Synonyms

KIAA1786 antibody

KIAA1906 antibody

transmembrane protein 132B antibody

Images

  • Western blot analysis of TMEM132B on different lysates with Rabbit anti-TMEM132B antibody (<a href="/products/0903-1" style="font-weight: bold;text-decoration: underline;">0903-1</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: SK-Br-3 cell lysate (20 µg/Lane)<br />Lane 3: U-87 MG cell lysate (20 µg/Lane)<br />Lane 4: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 5: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 119 kDa<br />Observed band size: 80/130 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/0903-1" style="font-weight: bold;text-decoration: underline;">0903-1</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TMEM132B on different lysates with Rabbit anti-TMEM132B antibody (0903-1) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: SK-Br-3 cell lysate (20 µg/Lane)
    Lane 3: U-87 MG cell lysate (20 µg/Lane)
    Lane 4: Mouse brain tissue lysate (40 µg/Lane)
    Lane 5: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 119 kDa
    Observed band size: 80/130 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0903-1) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"