TMEM2 Rabbit Polyclonal Antibody
Catalog# 0903-5
TMEM2 Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TMEM2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human TMEM2 aa 851-900 / 1,383.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 154 kDa
Positive Control
A549 cell lysate, PC-3M cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:5,000
Target
Function
Cell surface hyaluronidase that mediates the initial cleavage of extracellular high-molecular-weight hyaluronan into intermediate-size hyaluronan of approximately 10-5 kDa fragments. Very specific to hyaluronan; not able to cleave chondroitin sulfate or dermatan sulfate. Has an essential function in systemic hyaluronan catabolism and turnover and regulates cell adhesion and migration via hyaluronan degradation at focal adhesion sites (By similarity). Acts as a regulator of angiogenesis and heart morphogenesis by mediating degradation of extracellular hyaluronan, thereby regulating VEGF signaling (By similarity).
Background References
1. Yamamoto H et al. A Mammalian homolog of the zebrafish transmembrane protein 2 (TMEM2) is the long-sought-after cell surface hyaluronidase. J Biol Chem 292:7304-7313 (2017).
2. Scott D A et al. Refining the DFNB7-DFNB11 deafness locus using intragenic polymorphisms in a novel gene, TMEM2. Gene 246:265-274 (2000).
Sequence Similarity
Belongs to the CEMIP family.
Tissue Specificity
Widely expressed.
Subcellular Location
Cell membrane.
Synonyms
KIAA1412 antibody
TMEM 2 antibody
tmem2 antibody
TMEM2_HUMAN antibody
Transmembrane protein 2 antibody
Images
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Western blot analysis of TMEM2 on different lysates with Rabbit anti-TMEM2 antibody (0903-5) at 1/5,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: PC-3M cell lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (40 µg/Lane)
Lane 4: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 154 kDa
Observed band size: 200 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0903-5) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"