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Frizzled-5 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# 0905-3

Frizzled-5 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Frizzled-5 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Human Frizzled-5 aa 143-192 / 585.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, FC

Target Molecular Weight

Predicted band size: 64 kDa

Positive Control

Human ES cell, A549, LOVO, HepG2.

Conjugation

unconjugated

RRID

AB_3068708

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Frizzled-5 belongs to the G-protein coupled receptor Fz/Smo family, contains 585 amino acids. It is localized at the plasma membrane and also found at the Golgi. Fzds have a large extracellular N-terminal region containing a cysteine-rich domain (CRD), which is involved in binding to Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.

Background References

1. He X et al. A member of the Frizzled protein family mediating axis induction by Wnt-5A. Science 275:1652-1654 (1997).

2. Hsieh J.C et al. Biochemical characterization of Wnt-frizzled interactions using a soluble, biologically active vertebrate Wnt protein. Proc Natl Acad Sci USA 96:3546-3551 (1999).

Sequence Similarity

Belongs to the G-protein coupled receptor Fz/Smo family.

Post-translational Modification

Ubiquitinated by RNF43 and ZNRF3, leading to its degradation by the proteasome.

Subcellular Location

Cell membrane.Golgi apparatus. Membrane. Synapse.

UNIPROT

SwissProt: Q13467 Human

Synonyms

C2orf31 antibody

DKFZP434E2135 antibody

Frizzled 5 seven transmembrane spanning receptor antibody

Frizzled class receptor 5 antibody

Frizzled family receptor 5 antibody

Frizzled homolog 5 antibody

Frizzled-5 antibody

Frizzled5 antibody

Fz 5 antibody

Fz-5 antibody

Expand

Images

  • ICC staining Frizzled-5 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (<a href="/products/0905-3" style="font-weight: bold;text-decoration: underline;">0905-3</a>) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc&trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining Frizzled-5 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0905-3) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining Frizzled-5 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (<a href="/products/0905-3" style="font-weight: bold;text-decoration: underline;">0905-3</a>) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc&trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining Frizzled-5 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0905-3) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  • Flow cytometric analysis of Frizzled-5 was done on HepG2 cells. The cells were fixed, permeabilized and stained with Frizzled-5 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor&trade; 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution.

    Flow cytometric analysis of Frizzled-5 was done on HepG2 cells. The cells were fixed, permeabilized and stained with Frizzled-5 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution.

  • Western blot analysis of Frizzled-5 on different lysates with Rabbit anti-Frizzled-5 antibody (<a href="/products/0905-3" style="font-weight: bold;text-decoration: underline;">0905-3</a>) at 1/1,000 dilution.<br /><br />Lane 1: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 3 minutes; ECL: K1802<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/0905-3" style="font-weight: bold;text-decoration: underline;">0905-3</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 64 kDa<br />Observed band size: 70 kDa

    Western blot analysis of Frizzled-5 on different lysates with Rabbit anti-Frizzled-5 antibody (0905-3) at 1/1,000 dilution.

    Lane 1: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 3 minutes; ECL: K1802


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: 0905-3, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 64 kDa
    Observed band size: 70 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"