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FAT1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# 0905-4

FAT1 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

FAT1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Human Protocadherin Fat 1 aa 238-287 / 4,588.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 506 kDa

Positive Control

Recombinant protein

Conjugation

unconjugated

RRID

AB_3068709

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000-5,000

  • IHC-P

  • 1:100-1:400

Target

Function

Protocadherin FAT1 is a protein that in humans is encoded by the FAT1 gene. This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. This gene is expressed at high levels in a number of fetal epithelia. Transcript variants derived from alternative splicing and/or alternative promoter usage exist, but they have not been fully described. It has been shown that the EVH motifs in the cytoplasmic tail of mouse Fat1 interact with Ena/VASP and ablation of Fat1 by RNAi leads to decreased cell migration of rat epithelial cells. The cytoplasmic tail of Fat1 has also been shown to bind the transcriptional repressor Atrophin in rat vascular smooth muscle cells. At the carboxyl terminus of FAT1 lies a PDZ domain (PSD95/Dlg1/ZO-1) ligand motif (-HTEV). Zebrafish Fat1 was found to bind the protein scribble and regulate Hippo signalling. Using the human SHSY5Y cell line as a model of neuronal differentiation, human FAT1 was shown to regulate Hippo kinase components with loss of FAT1 leading to nucleocytoplasmic relocation of TAZ and enhanced transcription of the Hippo target gene CTGF. The same study also showed FAT1 was able to regulate TGF-beta signaling. FAT1 has been found to bind beta-catenin and regulate Wnt-signaling in colorectal cancer. Human FAT1 was found to bind glypican-3 (GPC3) and regulate cell migration in liver cancer cells.

Background References

1. "Molecular cloning and tissue expression of FAT, the human homologue of the Drosophila fat gene that is located on chromosome 4q34-q35 and encodes a putative adhesion molecule."Dunne J., Hanby A.M., Poulsom R., Jones T.A., Sheer D., Chin W.G., Da S.M., Zhao Q., Beverley P.C.L., Owen M.J.Genomics 30:207-223(1995)

2. "Protocadherin FAT1 binds Ena/VASP proteins and is necessary for actin dynamics and cell polarization" Marcus J Moeller, Abdulsalam Soofi, Gerald S Braun1, Xiaodong Li, Carsten Watzl2, Wilhelm Kriz1 and Lawrence B Holzman. The EMBO Journal 23:3769-3779(2004)

Tissue Specificity

Expressed in many epithelial and some endothelial and smooth muscle cells.

Post-translational Modification

Undergoes proteolytic cleavage. The extracellular domain is cleaved off and the cytoplasmic domain (about 400 AA) shuttles to the nucleus.

Subcellular Location

Cell membrane, Nucleus.

UNIPROT

SwissProt: Q14517 Human

Synonyms

Cadherin family member 7 antibody

Cadherin family member 7 precursor antibody

Cadherin ME5 antibody

Cadherin related tumor suppressor antibody

Cadherin related tumor suppressor homolog antibody

Cadherin related tumor suppressor homolog precursor antibody

Cadherin related tumor suppressor homolog precursor (FAT protein homolog) antibody

Cadherin-related tumor suppressor homolog antibody

CDHF 7 antibody

CDHF7 antibody

Expand

Images

  • Western blot analysis of FAT1 on recombinant Fat1 protein with Rabbit anti-FAT1 antibody (<a href="/products/0905-4" style="font-weight: bold;text-decoration: underline;">0905-4</a>) at 1/5,000 dilution.<br /><br />Predicted band size: 50 kDa<br />Observed band size: 50 kDa<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/0905-4" style="font-weight: bold;text-decoration: underline;">0905-4</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FAT1 on recombinant Fat1 protein with Rabbit anti-FAT1 antibody (0905-4) at 1/5,000 dilution.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0905-4) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using anti-FAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/0905-4" style="font-weight: bold;text-decoration: underline;">0905-4</a>, 1/100) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using anti-FAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0905-4, 1/100) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human Stomach tissue using anti-FAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/0905-4" style="font-weight: bold;text-decoration: underline;">0905-4</a>, 1/100) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human Stomach tissue using anti-FAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0905-4, 1/100) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation