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DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# 0912-1

DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody

Application

  • WB

  • IP

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide immune sequence is CDYKDDDDK.

Species Reactivity

Species independent

Validated Applications

WB, IP

Positive Control

DYKDDDDK Tag recombinant protein, N-terminal FLAG-tagged recombinant protein, C-terminal FLAG-tagged recombinant protein.

Conjugation

unconjugated

RRID

AB_3068715

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • 1-2μg/sample

Target

Function

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.

Background References

暂无

Synonyms

DDDDK epitope tag antibody

DDDK antibody

ddk antibody

DYKDDDDK antibody

DYKDDDDK epitope tag antibody

DYKDDDDK tag antibody

ECS epitope tag antibody

ECS tag antibody

Enterokinase Cleavage Site epitope tag antibody

Enterokinase Cleavage Site tag antibody

Expand

Images

  • Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/0912-1" style="font-weight: bold;text-decoration: underline;">0912-1</a>, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/0912-1" style="font-weight: bold;text-decoration: underline;">0912-1</a>, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Lane 1: N-terminal FLAG-tagged recombinant protein<br />Lane 2: C-terminal FLAG-tagged recombinant protein

    Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Lane 1: N-terminal FLAG-tagged recombinant protein
    Lane 2: C-terminal FLAG-tagged recombinant protein

  • DYKDDDDK Tag (FLAG) was immunoprecipitated from 0.2 mg L-929 transfected with FLAG-tagged CD5 cell lysate with <a href="/products/0912-1" style="font-weight: bold;text-decoration: underline;">0912-1</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/0912-1" style="font-weight: bold;text-decoration: underline;">0912-1</a> at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: L-929 transfected with FLAG-tagged CD5 cell lysate (input)<br />Lane 2: <a href="/products/0912-1" style="font-weight: bold;text-decoration: underline;">0912-1</a> IP in L-929 transfected with FLAG-tagged CD5 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/0912-1" style="font-weight: bold;text-decoration: underline;">0912-1</a> in L-929 transfected with FLAG-tagged CD5 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 3 seconds; ECL: K1801

    DYKDDDDK Tag (FLAG) was immunoprecipitated from 0.2 mg L-929 transfected with FLAG-tagged CD5 cell lysate with 0912-1 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using 0912-1 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: L-929 transfected with FLAG-tagged CD5 cell lysate (input)
    Lane 2: 0912-1 IP in L-929 transfected with FLAG-tagged CD5 cell lysate
    Lane 3: Rabbit IgG instead of 0912-1 in L-929 transfected with FLAG-tagged CD5 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 3 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation