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HRP Conjugated DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody

Usd: 339

Specification

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Catalog# 0912-3

HRP Conjugated DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Species independent

Conjugation

  • HRP

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HRP Conjugated DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide immune sequence is CDYKDDDDK.

Species Reactivity

Species independent

Validated Applications

WB

Positive Control

DYKDDDDK Tag recombinant protein

Conjugation

HRP

RRID

AB_3068717

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:10,000

Target

Function

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.

Background References

暂无

Synonyms

DDDDK epitope tag antibody

DDDK antibody

ddk antibody

DYKDDDDK antibody

DYKDDDDK epitope tag antibody

DYKDDDDK tag antibody

ECS epitope tag antibody

ECS tag antibody

Enterokinase Cleavage Site epitope tag antibody

Enterokinase Cleavage Site tag antibody

Expand

Images

  • Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates with Rabbit anti-DYKDDDDK Tag (FLAG) antibody (<a href="/products/0912-3" style="font-weight: bold;text-decoration: underline;">0912-3</a>) at 1/10,000 dilution.<br /><br />Lane 1: HeLa transfected with empty control cell lysate (20 µg/Lane)<br />Lane 2: HeLa transfected with FLAG-tagged Otx1 (C-terminal) cell lysate (10 µg/Lane)<br />Lane 3: 293T transfected with empty control cell lysate (20 µg/Lane)<br />Lane 4: 293T transfected with FLAG-tagged MYRF (N-terminal) cell lysate (40 µg/Lane)<br /><br />Exposure time: 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/0912-3" style="font-weight: bold;text-decoration: underline;">0912-3</a>) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight.

    Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates with Rabbit anti-DYKDDDDK Tag (FLAG) antibody (0912-3) at 1/10,000 dilution.

    Lane 1: HeLa transfected with empty control cell lysate (20 µg/Lane)
    Lane 2: HeLa transfected with FLAG-tagged Otx1 (C-terminal) cell lysate (10 µg/Lane)
    Lane 3: 293T transfected with empty control cell lysate (20 µg/Lane)
    Lane 4: 293T transfected with FLAG-tagged MYRF (N-terminal) cell lysate (40 µg/Lane)

    Exposure time: 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0912-3) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation